General Information

    • DRAMP ID

    • DRAMP18515

    • Peptide Name

    • A12L/A20L (V13KL peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from the framework peptide V13KL

    • Gene

    • Not found

    • Sequence

    • KWKSFLkTFKSLKKTVLHTLLKAISS

    • Sequence Length

    • 26

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.17158938]Gram-negative bacteria: Pseudomonas aeruginosa PAO1(MIC=62.5 μg/ml), Pseudomonas aeruginosa WR5 (MIC=125 μg/ml), Pseudomonas aeruginosa PAK (MIC=62.5 μg/ml), Pseudomonas aeruginosa PA14 (MIC=125 μg/ml), Pseudomonas aeruginosa M2 (MIC=62.5 μg/ml), Pseudomonas aeruginosa CP204 (MIC=62.5 μg/ml), The geometric mean of MIC values above six Gram-negative strains (MIC=78.7 μg/ml).
      • [Ref.22837667]Cancer cell: HeLa cell (IC50=1.71±0.07 μmol/L).

    • Hemolytic Activity

      • [Ref.22837667]MHC=8.0 μg/ml, MHC=5.20±0.02 μmol/L against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18515 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18515.

Physicochemical Information

    • Formula

    • C145H242N36O34

    • Absent Amino Acids

    • CDEGMNPQRY

    • Common Amino Acids

    • K

    • Mass

    • 3033.74

    • PI

    • 10.78

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +8

    • Boman Index

    • -2116

    • Hydrophobicity

    • -0.062

    • Aliphatic Index

    • 105

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 220

    • Polar Residues

    • 7

DRAMP18515

DRAMP18515 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria. A12L/A20L against HeLa cells. Leucine to replace Alanine at the position 12 and 20 on the peptide V13KL. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Role of peptide hydrophobicity in the mechanism of action of alpha-helical antimicrobial peptides.

    • Reference

    • Antimicrob Agents Chemother. 2007 Apr;51(4):1398-406.

    • Author

    • Chen Y, Guarnieri MT, Vasil AI, Vasil ML, Mant CT, Hodges RS.
  • ·Literature 2

    • Title

    • Role of helicity on the anticancer mechanism of action of cationic-helical peptides.

    • Reference

    • Int J Mol Sci. 2012;13(6):6849-62. doi: 10.3390/ijms13066849.

    • Author

    • Huang YB, He LY, Jiang HY, Chen YX.