General Information

    • DRAMP ID

    • DRAMP18520

    • Peptide Name

    • K14D/K22D (A12L/A20L peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from the framework peptide A12L/A20L

    • Gene

    • Not found

    • Sequence

    • KWKSFLkTFKSLKkTVLHTLLkAISS

    • Sequence Length

    • 26

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Anti-Cancer

    • Target Organism

      • [Ref.22837667]Cancer cell: HeLa cell (IC50=1.40±0.09 μmol/L)

    • Hemolytic Activity

      • [Ref.22837667]MHC=20.81±0.06 μmol/L against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18520 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18520.

Physicochemical Information

    • Formula

    • C145H242N36O34

    • Absent Amino Acids

    • CDEGMNPQRY

    • Common Amino Acids

    • K

    • Mass

    • 3033.74

    • PI

    • 10.78

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +8

    • Boman Index

    • -2116

    • Hydrophobicity

    • -0.062

    • Aliphatic Index

    • 105

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 220

    • Polar Residues

    • 7

DRAMP18520

DRAMP18520 chydropathy plot

Comments Information

    • Function

    • K14D/K22D against HeLa cells. D-Lysine replace L-Lysine at the position 14 and 22 on the polar face of peptide A12L/A20L. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Role of helicity on the anticancer mechanism of action of cationic-helical peptides.

    • Reference

    • Int J Mol Sci. 2012;13(6):6849-62. doi: 10.3390/ijms13066849.

    • Author

    • Huang YB, He LY, Jiang HY, Chen YX.