• • DRAMP ID

  • DRAMP18533

• • Peptide Name

  • V13KL (V681 peptide derivative)

• • Source

  • Synthetic construct

• • Family

  • Derived from the framework peptide V681

• • Gene

  • Not found

• • Sequence

  • KWKSFLKTFKSAKKTVLHTALKAISS

• • Sequence Length

  • 26

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.15677462]Gram-positive bacteria: Staphylococcus aureus 25923 (MIC=64.0 μg/ml), Staphylococcus aureus SAP0017 (MIC=64.0 μg/ml), Staphylococcus epidermidis C621 (MIC=5.0 μg/ml), Bacillus subtilis C971 (MIC=1.6 μg/ml), Enterococcus faecalis C625 (MIC=64.0 μg/ml), Corynebacterium xerosis C875 (MIC=1.3 μg/ml), The geometric mean of MIC values above six Gram-positive strains (MIC=11.8 μg/ml);
    • Gram-negative bacteria: Escherichia coli UB1005 (MIC=2.5 μg/ml), Escherichia coli DC2 (MIC=1.6 μg/ml), Salmonella typhimurium C587 (MIC=4.0 μg/ml), Salmonella typhimurium C610 (MIC=1.3 μg/ml), Pseudomonas aeruginosa H187 (MIC=8.0 μg/ml), Pseudomonas aeruginosa H188 (MIC=5.0 μg/ml), The geometric mean of MIC values above six Gram-negative strains (MIC=3.1 μg/ml).
    • [Ref.17158938]Gram-negative bacteria: Pseudomonas aeruginosa PAO1(MIC=31.3 μg/ml), Pseudomonas aeruginosa WR5 (MIC=250 μg/ml), Pseudomonas aeruginosa PAK (MIC=125 μg/ml), Pseudomonas aeruginosa PA14 (MIC=62.5 μg/ml), Pseudomonas aeruginosa M2 (MIC=31.3 μg/ml), Pseudomonas aeruginosa CP204 (MIC=7.8 μg/ml), The geometric mean of MIC values above six Pseudomonas aeruginosa strains (MIC=49.6 μg/ml)

• • Hemolytic Activity

  • • [Ref.17158938]MHC=250.0 μg/ml against human red blood cells

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Not included yet

• • N-terminal Modification

  • Not included yet

• • C-terminal Modification

  • Not included yet

• • Nonterminal Modifications and Unusual Amino Acids

  • Not included yet

• • Stereochemistry

  • Not included yet

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP18533_predicted_structure.pdb to download.

DRAMP18533

• • Function

  • Antibacterial activity against Gram-positive and Gram-negative bacteria. L-Lysine replace L-Valine at the position 13 on the non-polar face of peptide V681. Has hemolytic activity.

• ·Literature 1

• • Title

  • Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index.

• • Pubmed ID

  • 15677462

• • Reference

  • J Biol Chem. 2005 Apr 1;280(13):12316-29.

• • Author

  • Chen Y, Mant CT, Farmer SW, Hancock RE, Vasil ML, Hodges RS.

• ·Literature 2

• • Title

  • Role of peptide hydrophobicity in the mechanism of action of alpha-helical antimicrobial peptides.

• • Pubmed ID

  • 17158938

• • Reference

  • Antimicrob Agents Chemother. 2007 Apr;51(4):1398-406.

• • Author

  • Chen Y, Guarnieri MT, Vasil AI, Vasil ML, Mant CT, Hodges RS.