• DRAMP ID

    • DRAMP18540
    • Peptide Name

    • V13AL (V681 peptide derivative)
    • Source

    • Synthetic construct
    • Family

    • Derived from the framework peptide V681
    • Gene

    • Not found
    • Sequence

    • KWKSFLKTFKSAAKTVLHTALKAISS
    • Sequence Length

    • 26
    • UniProt Entry

    • No entry found
    • Protein Existence

    • Synthetic
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.15677462]Gram-positive bacteria: Staphylococcus aureus 25923 (MIC=8.0 μg/ml), Staphylococcus aureus SAP0017 (MIC=5.0 μg/ml), Staphylococcus epidermidis C621 (MIC=3.2 μg/ml), Bacillus subtilis C971 (MIC=2.0 μg/ml), Enterococcus faecalis C625 (MIC=16.0 μg/ml), Corynebacterium xerosis C875 (MIC=2.0 μg/ml), The geometric mean of MIC values above six Gram-positive strains (MIC=4.5 μg/ml);
      • Gram-negative bacteria: Escherichia coli UB1005 (MIC=2.5 μg/ml), Escherichia coli DC2 (MIC=2.5 μg/ml), Salmonella typhimurium C587 (MIC=6.4 μg/ml), Salmonella typhimurium C610 (MIC=2.5 μg/ml), Pseudomonas aeruginosa H187 (MIC=5.0 μg/ml), Pseudomonas aeruginosa H188 (MIC=6.4 μg/ml), The geometric mean of MIC values above six Gram-negative strains (MIC=3.8 μg/ml)
    • Hemolytic Activity

      • [Ref.15677462]MHC=31.2 μg/ml against human red blood cells
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Alpha helix
    • Structure Description

    • Not found
    • Helical Wheel Diagram

    • DRAMP18540 helical wheel diagram
    • PDB ID

    • None
    • Predicted Structure

    • Formula

    • C136H223N35O34
    • Absent Amino Acids

    • CDEGMNPQRY
    • Common Amino Acids

    • K
    • Mass

    • 2892.48
    • PI

    • 10.7
    • Basic Residues

    • 7
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 12
    • Net Charge

    • +7
    • Boman Index

    • -2002
    • Hydrophobicity

    • 0.004
    • Aliphatic Index

    • 86.54
    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min
    • Extinction Coefficient Cystines

    • 5500
    • Absorbance 280nm

    • 220
    • Polar Residues

    • 7

DRAMP18540

DRAMP18540 chydropathy plot
    • Function

    • Antibacterial activity against Gram-positive and Gram-negative bacteria. L-Alanine to replace L-Valine at the position 13 on the non-polar face of peptide V681. Has hemolytic activity.
  • ·Literature 1
    • Title

    • Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index.
    • Reference

    • J Biol Chem. 2005 Apr 1;280(13):12316-29.
    • Author

    • Chen Y, Mant CT, Farmer SW, Hancock RE, Vasil ML, Hodges RS.