General Information
-
DRAMP ID
- DRAMP18585
-
Peptide Name
- [Arg4,8]ccTP (ccTP peptide derivative)
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Source
- Synthetic construct
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Family
- Derived from the peptide ccTP
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Gene
- Not found
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Sequence
- KWCRCVCRRGICYCRCRG
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Sequence Length
- 18
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anitifungal
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Target Organism
-
- [Ref.10673369]Gram-positive bacteria: Staphylococcus aureus (MIC=1.3 μM in low-salt; MIC=0.3 μM in high-salt), Micrococcus luteus (MIC=0.5 μM in low-salt; MIC=0.7 μM in high-salt), Enterococcus faecalis (MIC=1.0 μM in low-salt; MIC=1.2 μM in high-salt);
- Gram-negative bacteria: Escherichia coli (MIC=9.8 μM in low-salt; MIC=2.0 μM in high-salt), Pseudomonas aeruginosa (MIC=8.0 μM in low-salt; MIC=5.3 μM in high-salt), Primula vulgaris (MIC=26.7 μM in low-salt; MIC=56.2 μM in high-salt), Klebsiella oxytoca (MIC=6.1 μM in low-salt; MIC=7.8 μM in high-salt);
- Fungi: Candida albicans (MIC=6.4 μM in low-salt; MIC=11.2 μM in high-salt), Candida kefyr (MIC=1.1 μM in low-salt; MIC=1.9 μM in high-salt), Candida tropicalis (MIC=2.8 μM in low-salt; MIC=4.2 μM in high-salt).
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Hemolytic Activity
-
- [Ref.10673369] EC25=910 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- No specific N-terminal
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C-terminal Modification
- No specific C-terminal
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys3 and Cys16,Cys5 and Cys14,Cys7 and Cys12.
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Stereochemistry
- L
-
Structure
- Beta tile
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP18585.
Physicochemical Information
-
Formula
- C89H149N35O20S6
Absent Amino Acids
- ADEFHLMNPQST
Common Amino Acids
- C
Mass
- 2221.74
PI
- 9.49
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 3
Net Charge
- +6
-
Boman Index
- -5944
Hydrophobicity
- -0.317
Aliphatic Index
- 37.78
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 7365
Absorbance 280nm
- 433.24
Polar Residues
- 9
DRAMP18585
Comments Information
Function
- Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi.
Literature Information
- ·Literature 1
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Title
- Marked increase in membranolytic selectivity of novel cyclic tachyplesins constrained with an antiparallel two-beta strand cystine knot framework.
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Pubmed ID
- 10673369
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Reference
- Biochem Biophys Res Commun. 2000 Jan 27;267(3):783-90.
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Author
- Tam JP, Lu YA, Yang JL.