General Information
-
DRAMP ID
- DRAMP18586
-
Peptide Name
- [Arg4,8,13]ccTP (ccTP peptide derivative)
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Source
- Synthetic construct
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Family
- Derived from the peptide ccTP
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Gene
- Not found
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Sequence
- KWCRCVCRRGICRCRCRG
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anitifungal
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Target Organism
-
- [Ref.10673369]Gram-positive bacteria: Staphylococcus aureus (MIC=1.7 μM in low-salt; MIC=1.3 μM in high-salt), Micrococcus luteus (MIC=0.5 μM in low-salt; MIC=0.8 μM in high-salt), Enterococcus faecalis (MIC=0.7 μM in low-salt; MIC=0.5 μM in high-salt);
- Gram-negative bacteria: Escherichia coli (MIC=5.5 μM in low-salt; MIC=1.2 μM in high-salt), Pseudomonas aeruginosa (MIC=3.9 μM in low-salt; MIC=4.7 μM in high-salt), Primula vulgaris (MIC=16.8 μM in low-salt; MIC=55.6 μM in high-salt), Klebsiella oxytoca (MIC=2.4 μM in low-salt; MIC=5.4 μM in high-salt);
- Fungi: Candida albicans (MIC=7.1 μM in low-salt; MIC=5.1 μM in high-salt), Candida kefyr (MIC=4.2 μM in low-salt; MIC=0.7 μM in high-salt), Candida tropicalis (MIC=1.9 μM in low-salt; MIC=4.8 μM in high-salt).
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Hemolytic Activity
-
- [Ref.10673369] EC25=920 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- No specific N-terminal
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C-terminal Modification
- No specific C-terminal
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys3 and Cys16,Cys5 and Cys14,Cys7 and Cys12.
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Stereochemistry
- L
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP18586.
Physicochemical Information
-
Formula
- C86H152N38O19S6
Absent Amino Acids
- ADEFHLMNPQSTY
Common Amino Acids
- CR
Mass
- 2214.76
PI
- 9.99
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 3
Net Charge
- +7
-
Boman Index
- -7422
Hydrophobicity
- -0.494
Aliphatic Index
- 37.78
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5875
Absorbance 280nm
- 345.59
Polar Residues
- 8
DRAMP18586
Comments Information
Function
- Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. Has hemolytic activity.
Literature Information
- ·Literature 1
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Title
- Marked increase in membranolytic selectivity of novel cyclic tachyplesins constrained with an antiparallel two-beta strand cystine knot framework.
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Pubmed ID
- 10673369
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Reference
- Biochem Biophys Res Commun. 2000 Jan 27;267(3):783-90.
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Author
- Tam JP, Lu YA, Yang JL.