General Information

    • DRAMP ID

    • DRAMP18621

    • Peptide Name

    • Temporin-PEa (Temporin-PE peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from the peptide Temporin-PE

    • Gene

    • Not found

    • Sequence

    • FLYIVAKLLSGLL

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anitifungal

    • Target Organism

      • [Ref.29191658]Gram-positive bacteria: Staphylococcus aureus (MIC=1.0 μM), Methicillin-resistant Staphylococcus aureus (MIC=2.0 μM), Enterococcus faecalis (MIC=2.0 μM);
      • Fungus: Candida albicans (MIC=2.0 μM).
      • Cancer cell lines: NCI-H157 (IC50=3.398 μM), U251MG (IC50=3.520 μM), PC-3 (IC50=27.62 μM), MDA-MB-435s (IC50=9.864 μM), HMEC-1 (IC50=40.30 μM).

    • Hemolytic Activity

      • [Ref.29191658]HC50=531.7 μM against horse red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18621 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18621.

Physicochemical Information

    • Formula

    • C73H120N14O16

    • Absent Amino Acids

    • CDEHMNPQRTW

    • Common Amino Acids

    • L

    • Mass

    • 1449.84

    • PI

    • 8.59

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +1

    • Boman Index

    • 3020

    • Hydrophobicity

    • 1.992

    • Aliphatic Index

    • 210

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 124.17

    • Polar Residues

    • 3

DRAMP18621

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive bacteria and fungi. Weak hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus.

    • Reference

    • Biochem Biophys Res Commun. 2018 Jan 22;495(4):2539-2546.

    • Author

    • Sang M, Wu Q, Xi X, Ma C, Wang L, Zhou M, Burrows JF, Chen T.