General Information

    • DRAMP ID

    • DRAMP18622

    • Peptide Name

    • Temporin-PEb (Temporin-PE peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from the peptide Temporin-PE

    • Gene

    • Not found

    • Sequence

    • FLPIVAKLLSGLLGRKKRRQRRR

    • Sequence Length

    • 23

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anitifungal

    • Target Organism

      • [Ref.29191658]Gram-positive bacteria: Staphylococcus aureus (MIC=1.0 μM), Methicillin-resistant Staphylococcus aureus (MIC=2.0 μM), Enterococcus faecalis (MIC=2.0 μM);
      • Gram-negative bacteria: Escherichia coli (MIC=2.0 μM);
      • Fungus: Candida albicans (MIC=1.0 μM).
      • Cancer cell lines: NCI-H157 (IC50=3.464 μM), U251MG (IC50=3.087 μM), PC-3 (IC50=3.051 μM), MDA-MB-435s (IC50=3.483 μM), HMEC-1 (IC50=9.976 μM).

    • Hemolytic Activity

      • [Ref.29191658]HC50=44.64 μM against horse red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18622 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C124H225N45O26

    • Absent Amino Acids

    • CDEHMNTWY

    • Common Amino Acids

    • R

    • Mass

    • 2762.44

    • PI

    • 12.7

    • Basic Residues

    • 9

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +9

    • Boman Index

    • -7488

    • Hydrophobicity

    • -0.57

    • Aliphatic Index

    • 118.7

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP18622

DRAMP18622 chydropathy plot

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus.

    • Reference

    • Biochem Biophys Res Commun. 2018 Jan 22;495(4):2539-2546.

    • Author

    • Sang M, Wu Q, Xi X, Ma C, Wang L, Zhou M, Burrows JF, Chen T.