General Information
-
DRAMP ID
- DRAMP18628
-
Peptide Name
- D6-K9L8W (D-amino acid substitution of K9L8W)
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Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
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Sequence
- KLKKLLKKWLKLLKKLLK
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.20363271]Gram-positive bacteria: Bacillus subtilis (MIC=1 μM), Staphylococcus epidermidis (MIC=4 μM), Staphylococcus aureus (MIC=2 μM);
- Gram-negative bacteria: Escherichia coli (MIC=4 μM), Pseudomonas aeruginosa (MIC=2 μM), Salmonella typhimurium (MIC=2 μM).
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Hemolytic Activity
-
- [Ref.20363271]HC50>800 μM against human red blood cells
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP18628.
Physicochemical Information
-
Formula
- C113H208N28O19
Absent Amino Acids
- ACDEFGHIMNPQRSTVY
Common Amino Acids
- K
Mass
- 2263.07
PI
- 10.9
Basic Residues
- 9
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +9
-
Boman Index
- -826
Hydrophobicity
- -0.311
Aliphatic Index
- 173.33
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 323.53
Polar Residues
- 0
DRAMP18628
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. D-Leucine replace L-Leucine at the position 6 and 12 on the peptide K9L8W, D-Lysine replace L-Lysine at the position 3,15 and 18 on the peptide K9L8W, D-Tryptophan replace L-Tryptophan at the position 9 on the peptide K9L8W. Weak hemolytic activity.
Literature Information
- ·Literature 1
-
Title
- Cell selectivity and anti-inflammatory activity of a Leu/Lys-rich alpha-helical model antimicrobial peptide and its diastereomeric peptides.
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Pubmed ID
- 20363271
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Reference
- Peptides. 2010 Jul;31(7):1251-61.
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Author
- Wang P, Nan YH, Yang ST, Kang SW, Kim Y, Park IS, Hahm KS, Shin SY.