General Information

    • DRAMP ID

    • DRAMP18648

    • Peptide Name

    • [Pro3,DLeu9]TL(3) (Temporin L peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from Temporin L

    • Gene

    • Not found

    • Sequence

    • FVPWFSKFLPRIL

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.28863356] Gram-positive bacterium: Bacillus megaterium Bm11 (MIC=3.125μM), Staphylococcus epidermidis ATCC 12228 (MIC=25μM);
      • Gram-negative bacteria: Acinetobacter baumannii ATCC 19606 (MIC=50μM), Escherichia coli D21 (MIC=50μM), Pseudomonas syringae pv tabaci 1918 (MIC=50μM);
      • Yeasts: Candida albicans ATCC 10231 (MIC=3.125μM), Candida crusei ATCC 6258 (MIC=3.125μM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • The analogues bearing a D residue showed a reduction in the percentage of helicity compared to the corresponding L analogues in the MMs

    • Helical Wheel Diagram

    • DRAMP18648 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18648.

Physicochemical Information

    • Formula

    • C86H124N18O15

    • Absent Amino Acids

    • ACDEGHMNQTY

    • Common Amino Acids

    • F

    • Mass

    • 1650.04

    • PI

    • 11

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • 620

    • Hydrophobicity

    • 0.877

    • Aliphatic Index

    • 112.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 1

DRAMP18648

Comments Information

    • Function

    • Antimicrobial activity against the Gram-negative bacteria, Gram-positive bacteria and Fungi. The position 9 of [Pro3,DLeu9]TL(3) is D-Leucine, D-Proline replace L-Proline at the position 10 on the peptide [Pro3,DLeu9]TL(2).

Literature Information

  • ·Literature 1

    • Title

    • Glycine-replaced derivatives of [Pro3,DLeu9]TL, a temporin L analogue: Evaluation of antimicrobial, cytotoxic and hemolytic activities

    • Reference

    • Eur J Med Chem. 2017 Oct 20;139:750-763.

    • Author

    • Merlino F, Carotenuto A, Casciaro B, Martora F, Loffredo MR, Di Grazia A, Yousif AM, Brancaccio D, Palomba L, Novellino E, Galdiero M, Iovene MR, Mangoni ML, Grieco P.