General Information
-
DRAMP ID
- DRAMP18658
-
Peptide Name
- Temporin-PE (Edible frogs, amphibians, animals)
-
Source
- Pelophylax kl.esculentus(Europe edible frog)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- FLPIVAKLLSGLL
-
Sequence Length
- 13
-
UniProt Entry
- A0A2H4WAJ8
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anitifungal, Anti-cancer, Anti-Gram-
-
Target Organism
-
- [Ref.29191658]Gram-positive bacteria: Staphylococcus aureus (MIC=2.0 μM), Methicillin-resistant Staphylococcus aureus (MIC=4.0 μM), Enterococcus faecalis (MIC=8.0 μM);
- Gram-negative bacteria: Escherichia coli (MIC=16.0 μM);
- Fungus: Candida albicans (MIC=4.0 μM).
- Cancer cell lines: NCI-H157 (IC50=34.56 μM), U251MG (IC50=25.13 μM), PC-3 (IC50=38.56 μM), MDA-MB-435s (IC50=33.23 μM), HMEC-1 (IC50=58.62 μM).
-
Hemolytic Activity
-
- [Ref.29191658]HC50=39.64 μM against horse red blood cells
-
Cytotoxicity
-
- Not included yet
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Alpha helix
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP18658.
Physicochemical Information
-
Formula
- C69H118N14O15
Absent Amino Acids
- CDEHMNQRTWY
Common Amino Acids
- L
Mass
- 1383.78
PI
- 8.75
Basic Residues
- 1
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +1
-
Boman Index
- 3034
Hydrophobicity
- 1.969
Aliphatic Index
- 210
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP18658
Comments Information
Function
- Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi.Has hemolytic activity.
Literature Information
- ·Literature 1
-
Title
- Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus.
-
Pubmed ID
- 29191658
-
Reference
- Biochem Biophys Res Commun. 2018 Jan 22;495(4):2539-2546.
-
Author
- Sang M, Wu Q, Xi X, Ma C, Wang L, Zhou M, Burrows JF, Chen T.