General Information

    • DRAMP ID

    • DRAMP18659

    • Peptide Name

    • YFGAP-OH(Yellowfin tuna GAPDH-related antimicrobial peptide; fish, animals)

    • Source

    • Yellowfin tuna (Thunnus albacares)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • VKVGINGFGRIGRLVTRAAFHGKKVEVVAIND

    • Sequence Length

    • 32

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.22771964]Gram-positive bacteria: Bacillus subtilis KCTC1021 (MIC=13.0 μg/ml), Bacillus subtilis RM125 (MIC=1.2 μg/ml), Micrococcus luteus ATCC9341 (MIC=6.5 μg/ml), Staphylococcus aureus RM4220 (MIC=125.0 μg/ml), Staphylococcus aureus KCTC1621 (MIC=11.0 μg/ml), Streptococcus iniae FP5229 (MIC=17.0 μg/ml);
      • Gram-negative bacteria: Escherichia coli D31 (MIC=6.2 μg/ml), Escherichia coli KCTC1116 (MIC=5.0 μg/ml), Escherichia coli ML35p (MIC=3.0 μg/ml), Enterobacter cloacae KCTC1685 (MIC=12.0 μg/ml), Pseudomonas aeroginosa KCTC2004 (MIC=3.7 μg/ml), Salmonella enterica KCTC2514 (MIC=3.1 μg/ml), Shigella flexneri KCTC2517 (MIC=5.0 μg/ml), Aeromonas hydrophila KCTC2358 (MIC=8.0 μg/ml), Vibrio parahemolyticus KCCM41664 (MIC=3.2 μg/ml)

    • Hemolytic Activity

      • [Ref.22771964]0.4% hemolysis at 100 μg/ml against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix and Beta strands

    • Structure Description

    • One Alpha helix and two parallel Beta strands

    • Helical Wheel Diagram

    • DRAMP18659 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18659.

Physicochemical Information

    • Formula

    • C154H258N48O40

    • Absent Amino Acids

    • CMPQSWY

    • Common Amino Acids

    • V

    • Mass

    • 3422.04

    • PI

    • 11

    • Basic Residues

    • 7

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 15

    • Net Charge

    • +5

    • Boman Index

    • -3744

    • Hydrophobicity

    • 0.263

    • Aliphatic Index

    • 112.5

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 8

DRAMP18659

DRAMP18659 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacterial.Weak hemolytic activity.

    • YFGAP-OH is the C-terminus amidated modification of YFGAP.

Literature Information

  • ·Literature 1

    • Title

    • Purification and characterization of YFGAP, a GAPDH-related novel antimicrobial peptide, from the skin of yellowfin tuna, Thunnus albacares.

    • Reference

    • Fish Shellfish Immunol. 2012 Oct;33(4):743-52. doi: 10.1016/j.fsi.2012.06.023.

    • Author

    • Seo JK, Lee MJ, Go HJ, Park TH, Park NG.