General Information

    • DRAMP ID

    • DRAMP18662

    • Peptide Name

    • Brevinin 21 (Brevinin-1E truncated peptide 21; Frogs, amphibians, animals)

    • Source

    • Rana esculenta

    • Family

    • Derived from the peptide Brevinin-1E

    • Gene

    • Not found

    • Sequence

    • LLAGLAANFLPKIFCKITRKC

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anitifungal, Anti-Gram-

    • Target Organism

      • [Ref.9748603]Gram-positive bacteria: Staphylococcus aureus ATCC 6538 (MIC=12.5 μg/ml), Methicillin resistant Staphylococcus aureus (MIC=25 μg/ml), Micrococcus luteus ATCC 9341 (MIC=6.25 μg/ml);
      • Gram-negative bacteria:Escherichia coli ATCC 2592 (MIC=12.5 μg/ml), Pseudomonas aeruginosa ATCC 9027 (MIC=12.5 μg/ml);
      • Fungus: Candida albicans ATCC 36232 (MIC=6.25 μg/ml).

    • Hemolytic Activity

      • [Ref.9748603]HD50=77 μg/ml(oxidized); HD50=110 μg/ml(reduced) against mouse red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP18662 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP18662.

Physicochemical Information

    • Formula

    • C108H182N28O24S2

    • Absent Amino Acids

    • DEHMQSVWY

    • Common Amino Acids

    • L

    • Mass

    • 2320.93

    • PI

    • 9.85

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +4

    • Boman Index

    • 363

    • Hydrophobicity

    • 0.848

    • Aliphatic Index

    • 125.71

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.25

    • Polar Residues

    • 5

DRAMP18662

DRAMP18662 chydropathy plot

Comments Information

    • Function

    • Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. Has hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Structure-activity analysis of brevinin 1E amide, an antimicrobial peptide from Rana esculenta.

    • Reference

    • Biochim Biophys Acta. 1998 Sep 8;1387(1-2):239-48.

    • Author

    • Kwon MY, Hong SY, Lee KH.