• DRAMP ID

    • DRAMP18665
    • Peptide Name

    • Mastoparan-L (MP-L; insects, arthropods, invertebrates, animals)
    • Source

    • Vespula lewisii (Korean yellow-jacket wasp) (Vespula flaviceps lewisii)
    • Family

    • Belongs to the tetradecapeptide family
    • Gene

    • Unknown
    • Sequence

    • INLKALAALAKKIL
    • Sequence Length

    • 14
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Anti-Gram+, Anti-Gram-, Antimicrobial
    • Target Organism

      • [Ref.27423268]Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=12.5 μM), Enterococcus faecalis ATCC 29212 (MIC=25 μM);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=50 μM), Klebsiella pneumoniae ATCC 13883 (MIC=12.5 μM), Acinetobacter baumannii ATCC 19606 (MIC=2.7 μM)
    • Hemolytic activity

      • [Ref.27423268]40% hemolysis at 10μM against rat red blood cells
    • Binding Target

    • Unknown
    • Structure

    • Alpha helix
    • Structure Description

    • Unknownn
    • PDB ID

    • 1D7N resolved by NMR.
    • DRAMP18665 helical wheel diagram
    • 1D7N-> 
    • Formula

    • C70H130N18O16
    • Absent Amino Acids

    • CDEFGHMPQRSTVWY
    • Common Amino Acids

    • AL
    • Mass

    • 171201
    • PI

    • 11.1
    • Basic Residues

    • 3
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 10
    • Boman Index

    • 1347
    • Hydrophobicity

    • 115.71
    • Aliphatic Index

    • 195.71
    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 1

DRAMP18665

    • Function

    • Mast cell degranulating peptide. Activates G proteins that couple to phospholipase C. May be able to switch from an in-plane to a transmembrane orientation in lipid bilayers. Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Has hemolytic activity.
    • PTM

    • Leucine amide at the position 14.
    • Tissue specificity

    • Expressed by the venom gland.
  • Literature 1
    • Reference

    • Biochim Biophys Acta. 2016 Nov;1858(11):2699-2708.
    • Author

    • Irazazabal LN, Porto WF, Ribeiro SM, Casale S, Humblot V, Ladram A, Franco OL.
    • Title

    • Selective amino acid substitution reduces cytotoxicity of the antimicrobial peptide mastoparan.