• DRAMP ID

    • DRAMP18666
    • Peptide Name

    • P1 (Pilosulin-1 1-20; Ant, insects, arthropods, invertebrates, animals)
    • Source

    • Myrmecia pilosula (Jumper ant)
    • Family

    • Derived from the peptide Pilosulin-1, Belongs to the pilosulin family.
    • Gene

    • Unknown
    • Sequence

    • GLGSVFGRLARILGRVIPKV
    • Sequence Length

    • 20
    • Evidence code

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anitifungal, Anti-Gram-
    • Target Organism

      • [Ref.15639237]Gram-positive bacteria: Staphylococcus aureus 710A (MIC=4 μM), Bacillus megaterium BM11 (MIC=2 μM);
      • Gram-negative bacteria: Escherichia coli ML-35 (MIC=2 μM), Pseudomonas aeruginosa ATCC27853 (MIC=8 μM), Salmonella typhimurium ATCC14028 (MIC=8 μM).
      • Drug resistant clinical isolates: Staphylococcus simulans 22 (MIC=2 μM), Micrococcus luteus (MIC=0.5 μM), Enterococcus spp. I-11305b (MIC=2 μM), Enterococcus spp. I-11054 (MIC=2 μM), Staphylococcus haemolyticus I-10925 (MIC=2 μM), Staphylococcus epidermidis LT1324 (MIC=2 μM), Staphylococcus aureus 5185 (MIC=2 μM), Staphylococcus aureus I-11574 (MIC=2 μM), Staphylococcus aureus LT1338 (MIC=3 μM), Staphylococcus aureus T1334 (MIC=1.5 μM), Citrobacter freundii I-11090 (MIC=2 μM), Klebsiella pneumoniae I-10910 (MIC=2 μM), Escherichia coli I-11276b (MIC=4 μM), Escherichia coli O-19592 (MIC=2 μM), Stenotrophomonas maltophilia O-16451 (MIC=2 μM), Stenotrophomonas maltophilia I-10717 (MIC=2 μM), Pseudomonas aeruginosa 4991 (MIC=4 μM), Pseudomonas aeruginosa I-10968 (MIC=4 μM), Candida albicans I-11301 (MIC=4 μM).
    • Hemolytic activity

      • [Ref.15639237]20% hemolysis at 10μM, 100% hemolysis at 100μM against human red blood cells
    • Binding Target

    • Unknown
    • Structure

    • Alpha helix
    • Structure Description

    • Unknownn
    • PDB ID

    • 5X3L resolved by NMR.
    • DRAMP18666 helical wheel diagram
    • 5X3L-> 
    • Formula

    • C97H170N30O22
    • Absent Amino Acids

    • CDEHMNQTWY
    • Common Amino Acids

    • G
    • Mass

    • 244769
    • PI

    • 12.81
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 10
    • Boman Index

    • -844
    • Hydrophobicity

    • 81
    • Aliphatic Index

    • 146
    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 0
    • Absorbance 280nm

    • 0
    • Polar Residues

    • 5

DRAMP18666

DRAMP18666 chydropathy plot
    • Function

    • Has strong cytotoxic and hemolytic activities. Is more potent against mononuclear leukocytes than against granulocytes. Antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. Adopts an alpha-helical structure.
    • Allergenic properties

    • Causes an allergic reaction in human. Binds to IgE.
  • Literature 1
    • Reference

    • Arch Biochem Biophys. 2005 Feb 15;434(2):358-64.
    • Author

    • Zelezetsky I, Pag U, Antcheva N, Sahl HG, Tossi A.
    • Title

    • Identification and optimization of an antimicrobial peptide from the ant venom toxin pilosulin.