General Information

    • DRAMP ID

    • DRAMP20771

    • Peptide Name

    • Acanthaporin (parasite, amoebozoa, protozoa, protists)

    • Source

    • Acanthamoeba culbertsoni

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GKCSVLKKVACAAAIAGAVAACGGIDLPCVLAALKAAEGCASCFCEDHCHGVCKDLHLC

    • Sequence Length

    • 59

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20771 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP20771.

Physicochemical Information

    • Formula

    • C244H404N70O72S10

    • Absent Amino Acids

    • MNQRTWY

    • Common Amino Acids

    • A

    • Mass

    • 5790.92

    • PI

    • 6.94

    • Basic Residues

    • 8

    • Acidic Residues

    • 5

    • Hydrophobic Residues

    • 27

    • Net Charge

    • +3

    • Boman Index

    • 1620

    • Hydrophobicity

    • 0.878

    • Aliphatic Index

    • 99.49

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 625

    • Absorbance 280nm

    • 10.78

    • Polar Residues

    • 18

DRAMP20771

DRAMP20771 chydropathy plot

Comments Information

    • Function

    • Acanthaporin is antimicrobially active against several Gram-positive and Gram-negative bacteria at pH 5.2 but is inactive at pH 7.4, reflecting a pH-dependent activity of the protein.

Literature Information

  • ·Literature 1

    • Title

    • Structure and function of a unique pore-forming protein from a pathogenic acanthamoeba.

    • Reference

    • Nat Chem Biol. 2013 Jan;9(1):37-42.

    • Author

    • Michalek M, Sönnichsen FD, Wechselberger R, Dingley AJ, Hung CW, Kopp A, Wienk H, Simanski M, Herbst R, Lorenzen I, Marciano-Cabral F, Gelhaus C, Gutsmann T, Tholey A, Grötzinger J, Leippe M.