General Information

    • DRAMP ID

    • DRAMP20776

    • Peptide Name

    • HaA4 (beetles,insects,animals)

    • Source

    • Harmonia axyridis (Multicolored Asian lady beetle)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IGGYCSWLRL

    • Sequence Length

    • 10

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.23124352] Gram-negative bacteria: Escherichia coli KACC 13821 (MIC=32 microM), Klebsiella pneumoniae KACC 11402 (MIC<64 microM), Pseudomonas aeruginosa KACC 10259 (MIC<64 microM), Pseudomonas syringae KACC 10659 (MIC=2 microM)；
      • Gram-positive bacteria: Staphylococcus aureus KACC 13257 (MIC<64 microM), MR Staphylococcus aureus CCARM 3001(MIC=32 microM), Enterococcus faecalis KACC 13807 (MIC=16 microM), Enterococcus hirae KACC 16328 ( MIC=32 microM), Staphylococcus epidermidis KACC 13234 (MIC=8 microM), Micrococcus luteus KACC 10488 (MIC = 4 microM), Streptococcus pyogenes KACC 11956 (MIC=32 microM)

    • Hemolytic Activity

      • [Ref.23124352] In the hemolytic assay, HaA4 peptide evidenced no hemolytic activity against mouse red blood cells, even at high concentrations, and thus suggesting that the peptide may not be detrimental to eukaryotic cells.

    • Cytotoxicity

      • [Ref.23124352] The cytotoxicity is not found in a dose dependent manner.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20776 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20776.

Physicochemical Information

    • Formula

    • C54H82N14O13S

    • Absent Amino Acids

    • ADEFHKMNPQTV

    • Common Amino Acids

    • GL

    • Mass

    • 1167.39

    • PI

    • 8.22

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +1

    • Boman Index

    • 179

    • Hydrophobicity

    • 0.63

    • Aliphatic Index

    • 117

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 776.67

    • Polar Residues

    • 5

DRAMP20776

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria. HaA4 is proved to have more potent activity against Staphylococcus epidermidis, M. luteus, and Pseudomonas syringae.

Literature Information

  • ·Literature 1

    • Title

    • Characterization and cDNA cloning of a defensin-like peptide, harmoniasin, from Harmonia axyridis.

    • Reference

    • J Microbiol Biotechnol. 2012 Nov;22(11):1588-90.

    • Author

    • Kim IW, Lee JH, Park HY, Kwon YN, Yun EY, Nam SH, Kim SR, Ahn MY, Hwang JS.