• DRAMP ID

    • DRAMP20776
    • Peptide Name

    • HaA4 (beetles,insects,animals)
    • Source

    • Harmonia axyridis (Multicolored Asian lady beetle)
    • Family

    • Unknown
    • Gene

    • Unknown
    • Sequence

    • IGGYCSWLRL
    • Sequence Length

    • 10
    • Swiss_Prot Entry

    • No entry found
    • Evidence code

    • Unknown
    • Biological Activity

    • Antibacterial, Antimicrobial, Anti-Gram+, Anti-Gram-
    • Target Organism

      • [Ref.23124352] Gram-negative bacteria: Escherichia coli KACC 13821 (MIC=32 microM), Klebsiella pneumoniae KACC 11402 (MIC<64 microM), Pseudomonas aeruginosa KACC 10259 (MIC<64 microM), Pseudomonas syringae KACC 10659 (MIC=2 microM);
      • Gram-positive bacteria: Staphylococcus aureus KACC 13257 (MIC<64 microM), MR Staphylococcus aureus CCARM 3001(MIC=32 microM), Enterococcus faecalis KACC 13807 (MIC=16 microM), Enterococcus hirae KACC 16328 ( MIC=32 microM), Staphylococcus epidermidis KACC 13234 (MIC=8 microM), Micrococcus luteus KACC 10488 (MIC = 4 microM), Streptococcus pyogenes KACC 11956 (MIC=32 microM)
    • Hemolytic activity

      • [Ref.23124352] In the hemolytic assay, HaA4 peptide evidenced no hemolytic activity against mouse red blood cells, even at high concentrations, and thus suggesting that the peptide may not be detrimental to eukaryotic cells.
    • Binding Target

    • Unknown
    • Structure

    • Unknown
    • Structure Description

    • Unknown
    • DRAMP20776 helical wheel diagram
    • Formula

    • C54H82N14O13S
    • Absent Amino Acids

    • ADEFHKMNPQTV
    • Common Amino Acids

    • GL
    • Mass

    • 1167.39
    • PI

    • 8.22
    • Basic Residues

    • 1
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 4
    • Boman Index

    • 179
    • Hydrophobicity

    • 63
    • Aliphatic Index

    • 117
    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 6990
    • Absorbance 280nm

    • 776.67
    • Polar Residues

    • 5

DRAMP20776

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria. HaA4 is proved to have more potent activity against Staphylococcus epidermidis, M. luteus, and Pseudomonas syringae.
  • Literature 1
    • Reference

    • J Microbiol Biotechnol. 2012 Nov;22(11):1588-90.
    • Author

    • Kim IW, Lee JH, Park HY, Kwon YN, Yun EY, Nam SH, Kim SR, Ahn MY, Hwang JS.
    • Title

    • Characterization and cDNA cloning of a defensin-like peptide, harmoniasin, from Harmonia axyridis.