General Information

    • DRAMP ID

    • DRAMP20777

    • Peptide Name

    • Cath-BF

    • Source

    • Bungarus fasciatus (Banded krait)

    • Family

    • Derived from three-finger toxin

    • Gene

    • Not found

    • Sequence

    • KRFKKFFRKLKKSVKKRAKEFFKKPRVIGVSIPF

    • Sequence Length

    • 34

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29392557] Gram-positive bacteria: MRSA PS-2 (MIC=64 μg/ml);
      • Gram-negative bacteria, MRSA PS-7 (MIC=64 μg/ml), MRSA PS-18 (MIC=64 μg/ml), MRSA PS-45 (MIC=64 μg/ml), MRSA PS-46 (MIC=128 μg/ml), MRSA PS-50 (MIC=64 μg/ml), MRSA PS-59 (MIC=128 μg/ml), MRSA PS-70 (MIC=32 μg/ml), MRSA PS-84 (MIC=128 μg/ml), MRSA PS-106 (MIC=128 μg/ml), MRSA PS-131 (MIC=64 μg/ml), MRSA PS-139 (MIC=64 μg/ml), MRSA PS-162 (MIC=64 μg/ml), MRSA PS-165 (MIC=32 μg/ml), Staphylococcus aureus ATCC 25923 (MIC=16 μg/ml), Staphylococcus aureus ATCC 33591 (MIC=256 μg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=8 μg/ml), Escherichia coli ATCC 51446 (MIC=16 μg/ml), Klebsiella pneumoniae ATCC 700603 (MIC=32 μg/ml), Klebsiella pneumoniae ATCC 13883 (MIC=4 μg/ml)

    • Hemolytic Activity

      • [Ref.29392557] 5% haemolysis at 1.0 μg/ml, 5% haemolysis at 2.0 μg/ml, 5% haemolysis at 4.0 μg/ml, 5% haemolysis at 8.0 μg/ml, 7% haemolysis at 16.0 μg/ml, 9% haemolysis at 31.2 μg/ml, 13% haemolysis at 62.5 μg/ml, 17% haemolysis at 125.0 μg/ml, 19% haemolysis at 250.0 μg/ml, 23% haemolysis at 500.0 μg/ml, 24% haemolysis at 1000.0 μg/ml against human red blood cells

    • Cytotoxicity

      • [Ref.29392557] The cell viability of HUVEC at the peptide concentration of 35.5 μg/ml, 71 μg/ml and 113 μg/ml is 127%, 100% and 76%.
      • The cell viability of H9c2 at the peptide concentration of 35.5, 71, and 113 μg/ml is 132, 100, and 80%.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20777 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20777.

Physicochemical Information

    • Formula

    • C203H335N57O39

    • Absent Amino Acids

    • CDHMNQTWY

    • Common Amino Acids

    • K

    • Mass

    • 4198.25

    • PI

    • 12.04

    • Basic Residues

    • 15

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +14

    • Boman Index

    • -8683

    • Hydrophobicity

    • -0.753

    • Aliphatic Index

    • 62.94

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP20777

DRAMP20777 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus.

    • Reference

    • J Microbiol. 2018 Feb;56(2):128-137.

    • Author

    • Tajbakhsh M, Karimi A, Tohidpour A, Abbasi N, Fallah F, Akhavan MM.