General Information

    • DRAMP ID

    • DRAMP20797

    • Peptide Name

    • Uperin 3.6 (Toad, amphibians, animals)

    • Source

    • Uperoleia mjobergii (Australian toadlet)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Uperin subfamily.

    • Gene

    • Not found

    • Sequence

    • GVIDAAKKVVNVLKNLF

    • Sequence Length

    • 17

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.10461748] Gram positive bacteria: Bacillus cereus (MIC=25 μg/ml), Leuconostoc lactis (MIC=3 μg/ml), Listeria innocua (MIC=50 μg/ml), Micrococcus luteus (MIC=50 μg/ml), Staphylococcus aureus (MIC=25 μg/ml), Staphylococcus epidermidis (MIC=12 μg/ml), Streptococcus uberis (MIC=12 μg/ml);
      • Gram-negative bacterium: Pasteurella haemolytica (MIC=25 μg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20797 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20797.

Physicochemical Information

    • Formula

    • C85H146N22O22

    • Absent Amino Acids

    • CEHMPQRSTWY

    • Common Amino Acids

    • V

    • Mass

    • 1828.23

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +2

    • Boman Index

    • -19

    • Hydrophobicity

    • 0.747

    • Aliphatic Index

    • 148.82

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP20797

Comments Information

    • Function

    • Antibacterial activity against B.cereus, L.lactis, L.innocua, M.luteus, S.aureus, S.epidermidis and S.uberis.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

    • Mass spectrometry

    • Molecular mass is 1826 Da from positions 1 - 17. Determined by FAB.

Literature Information

  • ·Literature 1

    • Title

    • The solution structure of uperin 3.6, an antibiotic peptide from the granular dorsal glands of the Australian toadlet, Uperoleia mjobergii.

    • Reference

    • J Pept Res. 1999 Aug;54(2):137-45.

    • Author

    • Chia BC, Carver JA, Mulhern TD, Bowie JH