General Information
-
DRAMP ID
- DRAMP20800
-
Peptide Name
- the K4 peptide (synthetic; Phe-rich >25%)
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Source
- the APD database-aided design.
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Family
- Not found
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Gene
- Not found
-
Sequence
- KKKKPLFGLFFGLF
-
Sequence Length
- 14
-
UniProt Entry
- No entry found
-
Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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Target Organism
-
- [Ref.19573572] Gram-negative bacteria: Escherichia coli (MIC=40-80 μg/ml or 24-48 μM), Vibrio alginolyticus (MIC=10-20 μg/ml or 6-12 μM), Vibrio harveyi (MIC=5-10 μg/ml or 3-6 μM).
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Hemolytic Activity
-
- [Ref.19573572] No significant hemolytic activity is observed at 10 and 40 μg/ml. Slight hemolysis (6.65%) is shown at 160 μg/ml.
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Cytotoxicity
-
- [Ref.19573572] The cell viability of CHO-K1 cells is 127%, 123%,78% and 10% at peptide concentrations of 10 μg/ml, 40 μg/ml, 160 μg/ml and 640 μg/ml
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20800.
Physicochemical Information
-
Formula
- C87H132N18O15
Absent Amino Acids
- ACDEHIMNQRSTVWY
Common Amino Acids
- FK
Mass
- 1670.12
PI
- 10.48
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +4
-
Boman Index
- 636
Hydrophobicity
- 0.329
Aliphatic Index
- 83.57
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP20800
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria. Have Hemolytic activity against rabbit erythrocytes. Have Cytotoxicity activity on CHO-K1 cells, the peptide is slightly cytotoxic at 160
Literature Information
- ·Literature 1
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Title
- KKKKPLFGLFFGLF: a cationic peptide designed to exert antibacterial activity
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Pubmed ID
- 19573572
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Reference
- Peptides. 2009 Sep;30(9):1608-12
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Author
- Duval E, Zatylny C, Laurencin M, Baudy-Floc'h M, Henry J.