General Information

    • DRAMP ID

    • DRAMP20824

    • Peptide Name

    • AM-CATH36

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLFKKLRRKIKKGFKKIFKRLPPIGVGVSIPLAGKR

    • Sequence Length

    • 36

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28089718] Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=21μg/ml), Staphylococcus aureus ATCC 33592 (MIC=21μg/ml);
      • Gram-negative bacteria:Acinetobacter baumannii ATCC 9955 (MIC=5.2μg/ml), Acinetobacter baumannii ATCC BAA-1794 (MIC=5.2μg/ml), Escherichia coli ATCC 4157 (MIC=140μg/ml), K. pneumoniae ATCC 33495 (MIC=5.2μg/ml), K. pneumoniae ATCC BAA-1705 (MIC=5.6μg/ml), Pseudomonas aeruginosa ATCC 9027 (MIC=5.2μg/ml), Pseudomonas aeruginosa ATCC BAA-2110 (MIC=5.2μg/ml)

    • Hemolytic Activity

      • [Ref.28089718] 0% hemolysis at 300μg/ml against sheep red cells

    • Cytotoxicity

      • [Ref.28089718] The survival rate of A549 human lung epithelial cells is 124% at a peptide concentration of 300 μg/ml

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix, random coil

    • Structure Description

    • NA-CATH has previously been shown to be random coil in water or buffer and

    • Helical Wheel Diagram

    • DRAMP20824 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20824.

Physicochemical Information

    • Formula

    • C194H337N57O38

    • Absent Amino Acids

    • CDEHMNQTWY

    • Common Amino Acids

    • K

    • Mass

    • 4076.17

    • PI

    • 12.49

    • Basic Residues

    • 13

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +13

    • Boman Index

    • -5014

    • Hydrophobicity

    • -0.247

    • Aliphatic Index

    • 105.56

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 6

DRAMP20824

DRAMP20824 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria

Literature Information

  • ·Literature 1

    • Title

    • Cathelicidin antimicrobial peptide from Alligator mississippiensis has antibacterial activity against multi-drug resistant Acinetobacter baumanii and Klebsiella pneumoniae

    • Reference

    • Dev Comp Immunol. 2017 May;70:135-144.

    • Author

    • Barksdale SM, Hrifko EJ, van Hoek ML.