General Information
-
DRAMP ID
- DRAMP20825
-
Peptide Name
- AM-CATH28
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Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KIKKGFKKIFKRLPPIGVGVSIPLAGKR
-
Sequence Length
- 28
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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Target Organism
-
- [Ref.28089718] Gram-negative bacteria:Acinetobacter baumannii ATCC 9955 (MIC=28μg/ml), Acinetobacter baumannii ATCC BAA-1794 (MIC=10μg/ml), K. pneumoniae ATCC 33495 (MIC=5.6μg/ml), K. pneumoniae ATCC BAA-1705 (MIC=28μg/ml), Pseudomonas aeruginosa ATCC 9027 (MIC=21μg/ml)
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Hemolytic Activity
-
- [Ref.28089718] 1% hemolysis at 300μg/ml against sheep red cells
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Cytotoxicity
-
- [Ref.28089718] The survival rate of A549 human lung epithelial cells is 122% at a peptide concentration of 300 μg/ml
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Alpha helix, random coil
-
Structure Description
- NA-CATH has previously been shown to be random coil in water or buffer and
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20825.
Physicochemical Information
-
Formula
- C147H255N41O30
Absent Amino Acids
- CDEHMNQTWY
Common Amino Acids
- K
Mass
- 3076.9
PI
- 12.05
Basic Residues
- 9
Acidic Residues
- 0
Hydrophobic Residues
- 11
Net Charge
- +9
-
Boman Index
- -2296
Hydrophobicity
- -0.075
Aliphatic Index
- 107.86
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 5
DRAMP20825
Comments Information
Function
- Antibacterial activity against the Gram-negative and Gram-positive bacteria
Literature Information
- ·Literature 1
-
Title
- Cathelicidin antimicrobial peptide from Alligator mississippiensis has antibacterial activity against multi-drug resistant Acinetobacter baumanii and Klebsiella pneumoniae
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Pubmed ID
- 28089718
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Reference
- Dev Comp Immunol. 2017 May;70:135-144.
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Author
- Barksdale SM, Hrifko EJ, van Hoek ML.