General Information
-
DRAMP ID
- DRAMP20827
-
Peptide Name
- TB_L1FK
-
Source
- Synthetic construct
-
Family
- Derived from TB
-
Gene
- Not found
-
Sequence
- FLPIVGLLKSLLK
-
Sequence Length
- 13
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.28443279] Gram-positive bacteria: S.aureus ATCC 33591 (MIC=6μM), Staphylococcus epidermidis (MIC=1.5μM);
- Gram-negative bacteria: Klebsiella pneumoniae ATCC BAA-1706 (MIC=6μM), Pseudomonas aeruginosa ATCC 27853 (MIC=6μM)
-
Hemolytic Activity
-
- [Ref.28443279] 8% hemolysis at 48μM, 38% hemolysis at 96μM against human red cells
-
Cytotoxicity
-
- [Ref.28443279] IC50 = 52 μM against PBMCs.
- IC50 = 59 μM against A549 cells
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20827.
Physicochemical Information
-
Formula
- C72H125N15O15
Absent Amino Acids
- ACDEHMNQRTWY
Common Amino Acids
- L
Mass
- 1440.88
PI
- 10
Basic Residues
- 2
Acidic Residues
- 0
Hydrophobic Residues
- 8
Net Charge
- +2
-
Boman Index
- 2298
Hydrophobicity
- 1.531
Aliphatic Index
- 202.31
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP20827
Comments Information
Function
- Antibacterial activity against the Gram-negative and Gram-positive bacteria,antifungal activity.
Literature Information
- ·Literature 1
-
Title
- Analogs of the Frog-skin Antimicrobial Peptide Temporin 1Tb Exhibit a Wider Spectrum of Activity and a Stronger Antibiofilm Potential as Compared to the Parental Peptide
-
Pubmed ID
- 28443279
-
Reference
- Front Chem. 2017 Apr 11;5:24
-
Author
- Grassi L, Maisetta G, Maccari G, Esin S, Batoni G.