General Information

    • DRAMP ID

    • DRAMP20832

    • Peptide Name

    • Polybia-MP1S-D8N

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IDWKKLLNAAKQIL

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC=0.8μM), Staphylococcus aureus (MIC=0.8μM), Staphylococcus epidermidis (MIC=37.5μM);
      • Gram-negative bacteria:Escherichia coli (MIC=50μM), Shigella dysenteriae (MIC=100μM), Klebsiella pneumoniae (MIC=37.5μM)

    • Hemolytic Activity

      • [Ref.29075946] 16.3% hemolysis at 12.5μM, 37.8% hemolysis at 25μM against human red cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • The oct-4-enyl hydrocarbon staple between Leu6 and Ala10

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20832 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20832.

Physicochemical Information

    • Formula

    • C78H132N20O19

    • Absent Amino Acids

    • CEFGHMPRSTVY

    • Common Amino Acids

    • KL

    • Mass

    • 1654.03

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +2

    • Boman Index

    • -700

    • Hydrophobicity

    • 0.064

    • Aliphatic Index

    • 153.57

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 423.08

    • Polar Residues

    • 1

DRAMP20832

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria, position 6 and 10 are cross-linked residues connected by the oct-4-enyl hydrocarbon staple

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial activity and stability of stapled helices of polybia-MP1

    • Reference

    • Arch Pharm Res. 2017 Dec;40(12):1414-1421.

    • Author

    • Luong HX, Kim DH, Lee BJ, Kim YW