• • DRAMP ID

  • DRAMP20833

• • Peptide Name

  • [Pro3,DLeu9]TL(1) (Temporin L peptide derivative)

• • Source

  • Synthetic construct

• • Family

  • Derived from Temporin L

• • Gene

  • Not found

• • Sequence

  • FVPWFSKFLGRIL

• • Sequence Length

  • 13

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.28863356] Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=3.125μM), Staphylococcus aureus Cowan I (MIC=12.5μM), Staphylococcus epidermidis ATCC 12228 (MIC=6.25μM);
    • Gram-negative bacteria: Acinetobacter baumannii ATCC 19606 (MIC=12.5μM), Escherichia coli D21 (MIC=12.5μM), Pseudomonas syringae pv tabaci 1918 (MIC=12.5μM), Pseudomonas aeruginosa ATCC 2785 (MIC=50μM);
    • Yeasts: Candida albicans ATCC 10231 (MIC=3.125μM), Candida crusei ATCC 6258 (3.125μM).

• • Hemolytic Activity

  • • [Ref.28863356] 21±4% hemolysis at 50μM, 7±3% hemolysis at 25μM, 6±3% hemolysis at 12.5μM, 3±2% hemolysis at 6.25μM, 1±1% hemolysis at 3.125μM against human red blood cells.
    • 20.63 ± 2.66% cytotoxic effect at 12.5μM, 57.52 ± 3.99% cytotoxic effect at 25μM, 91.58 ± 3.85% cytotoxic effect at 50 μM on HaCaT cells.

• • Cytotoxicity

  • • [Ref.28863356] The cytotoxic effects of the peptide are 20.63±2.66%, 57.52±3.99% and 91.58±3.85% at 12.5, 25 and 50 μM

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • Mixed (D-Leu9)

• • Structure

  • Alpha helix

• • Structure Description

  • The analogues bearing a D residue shows a reduction in the percentage of helicity compared to the corresponding L analogues in the MMs

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20833.

DRAMP20833

• • Function

  • Antimicrobial activity against the Gram-negative bacteria, Gram-positive bacteria and Fungi. The position 9 of [Pro3,DLeu9]TL(1) is D-Leucine.

• ·Literature 1

• • Title

  • Glycine-replaced derivatives of [Pro3,DLeu9]TL, a temporin L analogue: Evaluation of antimicrobial, cytotoxic and hemolytic activities

• • Pubmed ID

  • 28863356

• • Reference

  • Eur J Med Chem. 2017 Oct 20;139:750-761.

• • Author

  • Merlino F, Carotenuto A, Casciaro B, Martora F, Loffredo MR, Di Grazia A, Yousif AM, Brancaccio D, Palomba L, Novellino E, Galdiero M, Iovene MR, Mangoni ML, Grieco P.