• • DRAMP ID

  • DRAMP20838

• • Peptide Name

  • Pb-CATH4 bivittatus antimicrobial peptides peptide derivative

• • Source

  • Synthetic construct

• • Family

  • Derived from cathelicidin family

• • Gene

  • Not found

• • Sequence

  • TRSRWRRFIRGAGRFARRYGWRIAL

• • Sequence Length

  • 25

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.28630199]Gram-negative bacteria:Escherichia coli (ATCC 25922)(MIC=1μg/ml);Pseudomonas aeruginosa (ATCC 27853)(MIC=3μg/ml);S. typhimurium (ATCC 14028)(MIC=0.5μg/ml);
    • Gram-positive bacteria:Staphylococcus aureus (ATCC 29213)(MIC>128μg/ml);B. cereus (ATCC 10876)(MIC=10μg/ml);
    • Clinical isolates:Escherichia coli(MIC=4.7μg/ml);K. pneumoniae(MIC=1.18μg/ml)

• • Hemolytic Activity

  • • [Ref.28630199] 0% hemolysis at 4μg/ml against human red blood cell; 1.4 ± 0.4% hemolysis at 8μg/ml against human red blood cell; 5.1 ± 0.5% hemolysis at 16μg/ml against human red blood cell;7.3 ± 0.6% hemolysis at 32μg/ml against human red blood cell; 12.2 ± 0.7% hemolysis at 64μg/ml against human red blood cell

• • Cytotoxicity

  • • [Ref.28630199] The cell viability of Pk15(pig kidney fibroblasts) is 103.8%, 124.6%, 94.7%,55.2% and 53.9% at peptide concentrations of 5, 10, 20, 30 and 64 μg/ml.
    • The cell viability of HaCaT(human keratinocytes) is 106.9%, 94.6%, 66.2%, 58.1% and 51.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix

• • Structure Description

  • The peptide adopts arginine content of this peptide is 37.5%, and it contains two tryptophan residues (8.3%)

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20838.

DRAMP20838

• • Function

  • Antibacterial activity against the Gram-positive and Gram-negative bacteria

• ·Literature 1

• • Title

  • Genomewide Analysis of the Antimicrobial Peptides in Python bivittatus and Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity.

• • Pubmed ID

  • 28630199

• • Reference

  • Antimicrob Agents Chemother. 2017 Aug 24;61(9).

• • Author

  • Kim D, Soundrarajan N, Lee J, Cho HS, Choi M, Cha SY, Ahn B, Jeon H, Le MT, Song H, Kim JH, Park C.