General Information

    • DRAMP ID

    • DRAMP20868

    • Peptide Name

    • H4

    • Source

    • Synthetic construct

    • Family

    • Derived from BMAP-27 and OP-145

    • Gene

    • Not found

    • Sequence

    • KFKKLFKKLSPVIGKEFKRIVERIKRFLR

    • Sequence Length

    • 29

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29910626] Gram-positive bacteria: Staphylococcus aureus ATCC 29213 (MIC=20 μM), Staphylococcus aureus ATCC 33591 (MIC=10 μM), Staphylococcus aureus ATCC 43300 (MIC=10 μM), Staphylococcus aureus ATCC BAA41 (MIC=10 μM), Enterococcus faecalis ATCC 19433 (MIC=15 μM), Enterococcus faecalis ATCC BAA2365 (MIC=5 μM), Staphylococcus epidermidis ATCC 12228 (MIC=10 μM), Enterococcus faecium ATCC BAA2316 (MIC=5 μM);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=10 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=20 μM), Acinetobacter baumannii ATCC 19606 (MIC=10 μM), Klebsiella pneumoniae ATCC 13883 (MIC=2.5 μM), Pseudomonas aeruginosa ATCC BAA2114 (MIC=25 μM), Escherichia coli ATCC BAA2452 (MIC=10 μM)

    • Hemolytic Activity

      • [Ref.29910626] 0% haemolysis at 5 μM, 0% haemolysis at 10 μM, 0% haemolysis at 25 μM, 0% haemolysis at 40 μM, 0% haemolysis at 55 μM, 0% haemolysis at 75 μM, 0% haemolysis at 80 μM, 2.1% haemolysis at 100 μM against human red blood cells

    • Cytotoxicity

      • [Ref.29910626] IC50 = 61.9±0.18 μM against HEK293.
      • IC50 = 64.9±1.01 μM against Vero.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • The peptide adopts a conformational structure with 90 % alpha helix.

    • Helical Wheel Diagram

    • DRAMP20868 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20868.

Physicochemical Information

    • Formula

    • C174H295N49O35

    • Absent Amino Acids

    • ACDHMNQTWY

    • Common Amino Acids

    • K

    • Mass

    • 3633.56

    • PI

    • 11.62

    • Basic Residues

    • 12

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +10

    • Boman Index

    • -7064

    • Hydrophobicity

    • -0.5

    • Aliphatic Index

    • 100.69

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP20868

DRAMP20868 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Hybridization and antibiotic synergism as a tool for reducing the cytotoxicity of antimicrobial peptides.

    • Reference

    • Infect Drug Resist. 2018 Jun 1;11:835-847.

    • Author

    • Almaaytah A, Qaoud MT, Abualhaijaa A, Al-Balas Q, Alzoubi KH.