General Information
-
DRAMP ID
- DRAMP20874
-
Peptide Name
- Chensinin-1b
-
Source
- Synthetic construct
-
Family
- Derived from chensinin
-
Gene
- Not found
-
Sequence
- SKVWRHWRRFWHRAHRKL
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.29549839] Gram-positive bacteria: Staphylococcus aureus (MIC=3.13 μM), Bacillus cereus (MIC=3.13 μM), Streptococcus lactis (MIC=3.13 μM), Enterococcus faecalis (MIC=1.56 μM), Enterococcus faecium (MIC=3.13 μM);
- Gram-negative bacteria: Escherichia coli (MIC=6.25 μM), Pseudomonas aeruginosa (MIC=1.56 μM);
- cancer cells: HeLa cervical carcinoma (IC50=113.10μ±μ4.10 μM), MCF-7 breast carcinoma (IC50=51.85μ±μ0.88 μM)
-
Hemolytic Activity
-
- [Ref.29549839] HC50>500 μM against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Cell membrane (disrupted the bacterial cell membranes through a
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
-
Structure
- Alpha helix, beta sheet and Random coil
-
Structure Description
- The peptide adopts a random coil structure in water and presents an ordered alpha-helix structure with 11.96 % beta-strands and 28.06 % alpha-helices in TFE solution
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20874.
Physicochemical Information
-
Formula
- C119H176N44O20
Absent Amino Acids
- CDEGIMNPQTY
Common Amino Acids
- R
Mass
- 2542.99
PI
- 12.6
Basic Residues
- 10
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +10
-
Boman Index
- -8234
Hydrophobicity
- -1.711
Aliphatic Index
- 43.33
Half Life
-
- Mammalian:1.9 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 16500
Absorbance 280nm
- 970.59
Polar Residues
- 1
DRAMP20874
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria. Anticancer activities against HeLa cervical carcinoma and MCF-7 breast carcinoma.
Literature Information
- ·Literature 1
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Title
- Antimicrobial activity and self-assembly behavior of antimicrobial peptide chensinin-1b with lipophilic alkyl tails.
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Pubmed ID
- 29549839
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Reference
- Eur J Med Chem. 2018 Apr 25;150:546-558.
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Author
- Dong W, Liu Z, Sun L, Wang C, Guan Y, Mao X, Shang D.