General Information

    • DRAMP ID

    • DRAMP20877

    • Peptide Name

    • PA-C1b

    • Source

    • Synthetic construct

    • Family

    • Derived from Chensinin-1b

    • Gene

    • Not found

    • Sequence

    • SKVWRHWRRFWHRAHRKL

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29549839] Gram-positive bacteria: Staphylococcus aureus (MIC=12.5 μM), Bacillus cereus (MIC=6.25 μM), Streptococcus lactis (MIC=3.13 μM), Enterococcus faecalis (MIC= 3.13 μM), Enterococcus faecium (MIC= 6.25 μM);
      • Gram-negative bacteria: Escherichia coli (MIC=12.5 μM), Pseudomonas aeruginosa (MIC=6.25 μM);
      • cancer cells: HeLa cervical carcinoma (IC50=21.04μ±μ0.18 μM), A375 melanoma (IC50=18.15μ±μ0.21 μM), MCF-7 breast carcinoma (IC50=20.63μ±μ0.47 μM)

    • Hemolytic Activity

      • [Ref.29549839] HC50>500 μM against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Acylation (Ser1 conjugated with palmitic acid)

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix, Random coil

    • Structure Description

    • The peptide adopts a random coil structure in water and presents an ordered alpha-helix structure with 12.88 % beta-strands and 22.67 % alpha-helices in TFE solution

    • Helical Wheel Diagram

    • DRAMP20877 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20877.

Physicochemical Information

    • Formula

    • C119H176N44O20

    • Absent Amino Acids

    • CDEGIMNPQTY

    • Common Amino Acids

    • R

    • Mass

    • 2542.99

    • PI

    • 12.6

    • Basic Residues

    • 10

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +10

    • Boman Index

    • -8234

    • Hydrophobicity

    • -1.711

    • Aliphatic Index

    • 43.33

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 970.59

    • Polar Residues

    • 1

DRAMP20877

DRAMP20877 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria. Anticancer activities against HeLa cervical carcinoma, A375 melanoma and MCF-7 breast carcinoma. The peptide is a lipopeptide with CH3(CH2)6CO-.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial activity and self-assembly behavior of antimicrobial peptide chensinin-1b with lipophilic alkyl tails.

    • Reference

    • Eur J Med Chem. 2018 Apr 25;150:546-558.

    • Author

    • Dong W, Liu Z, Sun L, Wang C, Guan Y, Mao X, Shang D.