General Information

    • DRAMP ID

    • DRAMP20883

    • Peptide Name

    • Cath-A

    • Source

    • Synthetic construct

    • Family

    • Derived from three-finger toxin

    • Gene

    • Not found

    • Sequence

    • KRFKKFFRKLKKSVKKRKKEFKKKPRVIKVSIPF

    • Sequence Length

    • 34

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29392557] Gram-positive bacteria: MRSA PS-2 (MIC=64 μg/ml);
      • Gram-negative bacteria, MRSA PS-7 (MIC=64 μg/ml), MRSA PS-18 (MIC=32 μg/ml), MRSA PS-45 (MIC=32 μg/ml), MRSA PS-46 (MIC=32 μg/ml), MRSA PS-50 (MIC=32 μg/ml), MRSA PS-59 (MIC=64 μg/ml), MRSA PS-70 (MIC=32 μg/ml), MRSA PS-84 (MIC=128 μg/ml), MRSA PS-106 (MIC=32 μg/ml), MRSA PS-131 (MIC=128 μg/ml), MRSA PS-139 (MIC=32 μg/ml), MRSA PS-162 (MIC=128 μg/ml), MRSA PS-165 (MIC=32 μg/ml), Staphylococcus aureus ATCC 25923 (MIC=32 μg/ml), Staphylococcus aureus ATCC 33591 (MIC=128 μg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=8 μg/ml), Escherichia coli ATCC 51446 (MIC=16 μg/ml), Klebsiella pneumoniae ATCC 700603 (MIC=64 μg/ml), Klebsiella pneumoniae ATCC 13883 (MIC=16 μg/ml)

    • Hemolytic Activity

      • [Ref.29392557] 0% haemolysis at 1.0 μg/ml, 0% haemolysis at 2.0 μg/ml, 0% haemolysis at 4.0 μg/ml, 0% haemolysis at 8.0 μg/ml, 0% haemolysis at 16.0 μg/ml, 0% haemolysis at 31.2 μg/ml, 2% haemolysis at 62.5 μg/ml, 4% haemolysis at 125.0 μg/ml, 15% haemolysis at 250.0 μg/ml, 23% haemolysis at 500.0 μg/ml, 53% haemolysis at 1000.0 μg/ml against human red blood cells

    • Cytotoxicity

      • [Ref.29392557] The cell viability of HUVEC is 132%, 99% and 79% at peptide concentrations of 35.5, 71 and 113 μg/ml .
      • The cell viability of H9c2is 129%, 97%, and 80% at peptide concentrations of 35.5, 71, and 113 μg/ml .

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20883 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20883.

Physicochemical Information

    • Formula

    • C207H354N60O39

    • Absent Amino Acids

    • ACDGHMNQTWY

    • Common Amino Acids

    • K

    • Mass

    • 4307.47

    • PI

    • 12.05

    • Basic Residues

    • 18

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +17

    • Boman Index

    • -10921

    • Hydrophobicity

    • -1.221

    • Aliphatic Index

    • 60

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP20883

DRAMP20883 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • The antimicrobial potential of a new derivative of cathelicidin from Bungarus fasciatus against methicillin-resistant Staphylococcus aureus.

    • Reference

    • J Microbiol. 2018 Feb;56(2):128-137.

    • Author

    • Tajbakhsh M, Karimi A, Tohidpour A, Abbasi N, Fallah F, Akhavan MM.