General Information

    • DRAMP ID

    • DRAMP20887

    • Peptide Name

    • NCP-2 (CTX-1 peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from CTX-1

    • Gene

    • Not found

    • Sequence

    • KLIPILSKTIPAGKNLFYKI

    • Sequence Length

    • 20

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.29364903] Gram-positive bacteria: Staphylococcus aureus ATCC 22953 (MBC=50 μg/ml), Streptococcus agalactiae ATCC 13813 (MBC=12.5 μg/ml);
      • Gram-negative bacteria: Escherichia coli ATCC 25922 (MBC=12.5 μg/ml), Pseudomonas aeruginosa ATCC 27853 (MBC=12.5 μg/ml), Acinetobacter baumannii (herpetological, cloacal) (MBC=1.6 μg/ml), Acinetobacter baumannii (ornithological, cloacal) (MBC=50 μg/ml), Acinetobacter baumannii (human, urinary) (MBC=1.6 μg/ml), Moraxella catarrhalis ATCC 25238 (MBC=1.6 μg/ml);
      • Fungi: Candida albicans ATCC 10231 (MBC=50 μg/mL), Malassezia pachydermatis DSMZ 6172 (MBC=6.3 μg/mL)

    • Hemolytic Activity

      • [Ref.29364903] 2% haemolysis at 12.5 μg/ml, 8% haemolysis at 100.0 μg/ml against sheep red blood cells

    • Cytotoxicity

      • [Ref.29364903] The cell death rate of Madin-Darby Bovine Kidney(MDBK) cells is 3 % and 5% at peptide concentrations of 12.5 and 100 μg/ml

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • The peptide adopts a conformational structure with 20 % alpha helix, 25 % beta sheet and 35 % random coil in the presence of DMPE-DMPG mixed vesicles.

    • Helical Wheel Diagram

    • DRAMP20887 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20887.

Physicochemical Information

    • Formula

    • C110H185N25O25

    • Absent Amino Acids

    • CDEHMQRVW

    • Common Amino Acids

    • IK

    • Mass

    • 2257.83

    • PI

    • 10.18

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • 522

    • Hydrophobicity

    • 0.425

    • Aliphatic Index

    • 141.5

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 78.42

    • Polar Residues

    • 5

DRAMP20887

DRAMP20887 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria. Antifungal activity.

Literature Information

  • ·Literature 1

    • Title

    • Novel Naja atra cardiotoxin 1 (CTX-1) derived antimicrobial peptides with broad spectrum activity.

    • Reference

    • PLoS One. 2018 Jan 24;13(1):e0190778.

    • Author

    • Sala A, Cabassi CS, Santospirito D, Polverini E, Flisi S, Cavirani S, Taddei S.