General Information
-
DRAMP ID
- DRAMP20889
-
Peptide Name
- VT18-KKLV (VT18 peptide derivative)
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Source
- Synthetic construct
-
Family
- Derived from VT18
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Gene
- Not found
-
Sequence
- VYPFMWGGAYCFCKAKLV
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.29277569] Gram-positive bacteria: Streptococcus pneumoniae ATCC 700669 (MIC=52 μg/ml), Streptococcus pneumoniae ATCC 49619 (MIC=105 μg/ml), Streptococcus pneumoniae ATCC 700677 (MIC=76 μg/ml)
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Hemolytic Activity
-
- [Ref.29277569] 0.5% hemolysis at 5 μg/ml, 1.0% hemolysis at 10μg/ml, 2.0% hemolysis at 20 μg/ml, 2.5% hemolysis at 50 μg/ml, 3.0% hemolysis at 100 μg/ml, 5.0% hemolysis at 250 μg/ml, 7.0% hemolysis at 500 μg/ml against human red blood cells
-
Cytotoxicity
-
- [Ref.29277569] The cell viability of NL-20 normal lung cell line is not affected (<20%) below 50 μg/ml.
- The cell viability of NL-20 is 82%, 44%, 17% and 13% at peptide concentrations of 50, 100, 250 and 500 μg/ml.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Double-stranded
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20889.
Physicochemical Information
-
Formula
- C101H143N21O21S3
Absent Amino Acids
- DEHINQRST
Common Amino Acids
- ACFGKVY
Mass
- 2083.55
PI
- 8.82
Basic Residues
- 2
Acidic Residues
- 0
Hydrophobic Residues
- 8
Net Charge
- +2
-
Boman Index
- 2032
Hydrophobicity
- 0.811
Aliphatic Index
- 65
Half Life
-
- Mammalian:100 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 8605
Absorbance 280nm
- 506.18
Polar Residues
- 6
DRAMP20889
Comments Information
Function
- Antibacterial activity against the Gram-positive bacteria.
Literature Information
- ·Literature 1
-
Title
- Membrane permeabilization design of antimicrobial peptides based on chikungunya virus fusion domain scaffold and its antibacterial activity against gram-positive Streptococcus pneumoniae in respiratory infection.
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Pubmed ID
- 29277569
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Reference
- Biochimie. 2018 Mar;146:139-147.
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Author
- Yang R, Zhang G, Zhang F, Li Z, Huang C.