General Information

    • DRAMP ID

    • DRAMP20891

    • Peptide Name

    • cVT18-CAKKLV (VT18 peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from VT18

    • Gene

    • Not found

    • Sequence

    • VYPFCWGGAYAFCKAKLV

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • [Ref.29277569] Gram-positive bacteria: Streptococcus pneumoniae ATCC 700669 (MIC=35 μg/ml), Streptococcus pneumoniae ATCC 49619 (MIC=67 μg/ml), Streptococcus pneumoniae ATCC 700677 (MIC=49 μg/ml)

    • Hemolytic Activity

      • [Ref.29277569] 0.5% hemolysis at 5 μg/ml, 0.5% hemolysis at 10 μg/ml, 0.6% hemolysis at 20 μg/ml, 1.0% hemolysis at 50 μg/ml, 1.9% hemolysis at 100 μg/ml, 2.5% hemolysis at 250 μg/ml, 3.5% hemolysis at 500 μg/ml against human red blood cell

    • Cytotoxicity

      • [Ref.29277569] The cell viability of NL-20 normal lung cell line is not affected (<20%) below 50 μg/ml.
      • The cell viability of NL-20 is 81%, 73%, 34% and 19% at peptide concentrations of 50, 100, 250 and 500 μg/ml.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys5 and Cys13.

    • Stereochemistry

    • L

    • Structure

    • Double-stranded

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20891 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20891.

Physicochemical Information

    • Formula

    • C99H139N21O21S2

    • Absent Amino Acids

    • DEHIMNQRST

    • Common Amino Acids

    • A

    • Mass

    • 2023.44

    • PI

    • 8.82

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • 1978

    • Hydrophobicity

    • 0.806

    • Aliphatic Index

    • 70.56

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 8605

    • Absorbance 280nm

    • 506.18

    • Polar Residues

    • 6

DRAMP20891

DRAMP20891 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive bacteria. The positions 4 and 13 of cVT18-CAKKLV have a disulfide bond to form a cyclic peptide.

Literature Information

  • ·Literature 1

    • Title

    • Membrane permeabilization design of antimicrobial peptides based on chikungunya virus fusion domain scaffold and its antibacterial activity against gram-positive Streptococcus pneumoniae in respiratory infection.

    • Reference

    • Biochimie. 2018 Mar;146:139-147.

    • Author

    • Yang R, Zhang G, Zhang F, Li Z, Huang C.