General Information

    • DRAMP ID

    • DRAMP20941

    • Peptide Name

    • [Pro3,DLeu9]TL(8) (Temporin L peptide derivative)

    • Source

    • Synthetic construct

    • Family

    • Derived from Temporin L

    • Gene

    • Not found

    • Sequence

    • FVPWFSKFLKRIL

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.28863356] Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=3.125μM), Staphylococcus aureus Cowan I (MIC=6.25μM), Staphylococcus epidermidis ATCC 12228 (MIC=3.125μM);
      • Gram-negative bacteria: Acinetobacter baumannii ATCC 19606 (MIC=3.125μM), Escherichia coli D21 (MIC=12.5μM), Pseudomonas syringae pv tabaci 1918 (MIC=6.25μM), Pseudomonas aeruginosa ATCC 2785 (MIC=12.5μM);
      • Yeasts: Candida albicans ATCC 10231 (MIC=1.56μM), Candida crusei ATCC 6258 (MIC=1.56μM)

    • Hemolytic Activity

      • [Ref.28863356]49.30 ± 2.88% cytotoxic effect at 12.5 μM, 66.86 ± 2.81% cytotoxic effect at 25 μM, 96.79 ± 1.24% cytotoxic effect at 50 μM on HaCaT cells.

    • Cytotoxicity

      • [Ref.28863356] The cytotoxic effect is 49.30±2.88%, 66.86±2.81% and 96.79±1.24% at peptide concentrations of 12.5, 25 and 50 μM

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • Mixed (D-Leu9)

    • Structure

    • Alpha helix

    • Structure Description

    • The analogues bearing a D residue showed a reduction in the percentage of helicity compared to the corresponding L analogues in the MMs

    • Helical Wheel Diagram

    • DRAMP20941 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20941.

Physicochemical Information

    • Formula

    • C87H129N19O15

    • Absent Amino Acids

    • ACDEGHMNQTY

    • Common Amino Acids

    • F

    • Mass

    • 1681.1

    • PI

    • 11.17

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +3

    • Boman Index

    • 65

    • Hydrophobicity

    • 0.7

    • Aliphatic Index

    • 112.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 1

DRAMP20941

Comments Information

    • Function

    • Antimicrobial activity against the Gram-negative bacteria, Gram-positive bacteria and Fungi. The position 9 of [Pro3,DLeu9]TL(8) is D-Leucine, L-Lysine replace L-Proline at the position 10 on the peptide [Pro3,DLeu9]TL(2).

Literature Information

  • ·Literature 1

    • Title

    • Glycine-replaced derivatives of [Pro3,DLeu9]TL, a temporin L analogue: Evaluation of antimicrobial, cytotoxic and hemolytic activities

    • Reference

    • Eur J Med Chem. 2017 Oct 20;139:750-761.

    • Author

    • Merlino F, Carotenuto A, Casciaro B, MartorMerlino F, Carotenuto A, Casciaro B, Martora F, Loffredo MR, Di Grazia A, Yousif AM, Brancaccio D, Palomba L, Novellino E, Galdiero M, Iovene MR, Mangoni ML, Grieco P.