General Information

    • DRAMP ID

    • DRAMP20945

    • Peptide Name

    • Recombinant Cecropin A (1–8)–LL37 (17–30) (C–L)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • KWKLFKKIFKRIVQRIKDFLRN

    • Sequence Length

    • 22

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.29925795] Gram-negative bacteria: Escherichia coli CVCC 245 (MIC=7.2 ± 0.14 μg/mL);
      • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (MIC=2.2 ± 0.05 μg/mL),L. mono. CVCC 1599 (MIC=2.1 ± 0.03 μg/mL)

    • Hemolytic Activity

      • [Ref.29925795] 50% hemolysis at 221 ± 3.45μg/ml against sheep red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20945 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20945.

Physicochemical Information

    • Formula

    • C140H230N40O27

    • Absent Amino Acids

    • ACEGHMPSTY

    • Common Amino Acids

    • K

    • Mass

    • 2905.62

    • PI

    • 11.76

    • Basic Residues

    • 9

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +8

    • Boman Index

    • -5905

    • Hydrophobicity

    • -0.664

    • Aliphatic Index

    • 101.82

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 261.9

    • Polar Residues

    • 1

DRAMP20945

DRAMP20945 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative bacteria and Gram-positive bacteria

Literature Information

  • ·Literature 1

    • Title

    • Expression, Purification, and Characterization of a Novel Hybrid Peptide with Potent Antibacterial Activity

    • Reference

    • Molecules. 2018 Jun 20;23(6). pii: E1491.

    • Author

    • Wei X, Wu R, Zhang L, Ahmad B, Si D, Zhang R.