General Information

    • DRAMP ID

    • DRAMP20950

    • Peptide Name

    • [E]4-VmCT1-NH2 VmCT1 petide derivative

    • Source

    • Synthetic construct

    • Family

    • Belongs to the scorpion NDBP 9 family

    • Gene

    • Not found

    • Sequence

    • FLGELWNVAKSVF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

      • [Ref.27912176] Gram-negative bacteria: Pseudomonas aeruginosa (ATCC 27853)(MIC=3.12μmol/L);Escherichia coli (D31)(MIC=50μmol/L);Serratia marcescens (ATCC 4112)(MIC=3.12μmol/L);
      • Gram-positive bacteria: Micrococcus luteus (A270)(MIC=25μmol/L);Staphylococcus aureus (ATCC 29213)(MIC=50μmol/L);Staphylococcus epidermidis (ATCC 12228)(MIC=50μmol/L);
      • Fungi:Candida albicans MDM8(MIC=50μmol/L);Candida tropicalis (IOC 4560)(MIC=12.5μmol/L);
      • Cryptococcus neoformans(MIC=12.5μmol/L)

    • Hemolytic Activity

      • [Ref.27912176] MHC=25μmol/L against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Helical structures

    • Structure Description

    • The peptide adopts Helical fraction 0.02 in water;0.15 in TFE/Water;0.17 in POPC;0.20 in POPC:POPG;0.24 in POPC:DOPE;

    • Helical Wheel Diagram

    • DRAMP20950 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20950.

Physicochemical Information

    • Formula

    • C74H108N16O18

    • Absent Amino Acids

    • CDHIMPQRTY

    • Common Amino Acids

    • FLV

    • Mass

    • 1509.77

    • PI

    • 6

    • Basic Residues

    • 1

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • 0

    • Boman Index

    • 656

    • Hydrophobicity

    • 0.8

    • Aliphatic Index

    • 112.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 458.33

    • Polar Residues

    • 3

DRAMP20950

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria and fungi. It showed a diminished antimicrobial activity against all the microorganisms tested, besides that it did not present activity at the range of concentrations analyzed against Escherichia coli SBS 363, E. cloacae, Bacillus subtilis, C. parapsilosis, A. niger and C. herbarum.

Literature Information

  • ·Literature 1

    • Title

    • Novel designed VmCT1 analogs with increased antimicrobial activity.

    • Reference

    • Eur J Med Chem. 2017 Jan 27;126:456-463.

    • Author

    • Pedron CN, Torres MT, Lima JADS, Silva PI, Silva FD, Oliveira VX.