General Information

    • DRAMP ID

    • DRAMP20952

    • Peptide Name

    • [W]9-VmCT1-NH2 VmCT1 petide derivative

    • Source

    • Synthetic construct

    • Family

    • Belongs to the scorpion NDBP 11 family

    • Gene

    • Not found

    • Sequence

    • FLGALWNVWKSVF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial

    • Target Organism

      • [Ref.27912176] Gram-negative bacteria: Escherichia coli (SBS 363)(MIC=1.56μmol/L);Pseudomonas aeruginosa (ATCC 27853)(MIC=0.78μmol/L);Escherichia coli (D31)(MIC=1.56μmol/L);Serratia marcescens (ATCC 4112)(MIC=1.56μmol/L);Enterobacter cloacae (beta-12)(MIC=6.52μmol/L);
      • Gram-positive bacteria: Micrococcus luteus (A270)(MIC=1.56μmol/L);Staphylococcus aureus (ATCC 29213)(MIC=6.25μmol/L);Bacillus subtilis (ATCC 6633)(MIC=3.12μmol/L);Staphylococcus epidermidis (ATCC 12228)(MIC=1.56μmol/L);
      • Fungi: Candida albicans MDM8(MIC=6.25μmol/L);Candida tropicalis (IOC 4560)(MIC=50μmol/L);Candida parapsilosis (IOC 4564)(MIC=50μmol/L);
      • Aspergillus niger(MIC=50μmol/L);
      • Cladosporium herbarum (ATCC 26362)(MIC=50μmol/L);
      • Cryptococcus neoformans(MIC=3.12μmol/L)

    • Hemolytic Activity

      • [Ref.27912176]MHC=1.60μmol/L against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Helical structures

    • Structure Description

    • The peptide adopts Helical fraction 0.02 in water;0.11 in TFE/Water;0.30 in POPC;0.11 in POPC:POPG;0.24 in POPC:DOPE;

    • Helical Wheel Diagram

    • DRAMP20952 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20952.

Physicochemical Information

    • Formula

    • C80H111N17O16

    • Absent Amino Acids

    • CDEHIMPQRTY

    • Common Amino Acids

    • FLVW

    • Mass

    • 1566.87

    • PI

    • 8.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +1

    • Boman Index

    • 1570

    • Hydrophobicity

    • 1

    • Aliphatic Index

    • 112.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 916.67

    • Polar Residues

    • 3

DRAMP20952

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria and fungi.It antimicrobial activities are increased or preserved when compared to VmCT1.

Literature Information

  • ·Literature 1

    • Title

    • Novel designed VmCT1 analogs with increased antimicrobial activity.

    • Reference

    • Eur J Med Chem. 2017 Jan 27;126:456-463.

    • Author

    • Pedron CN, Torres MT, Lima JADS, Silva PI, Silva FD, Oliveira VX.