General Information
-
DRAMP ID
- DRAMP20953
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Peptide Name
- [E]4[W]9-VmCT1-NH2 VmCT1 petide derivative
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Source
- Synthetic construct
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Family
- Belongs to the scorpion NDBP 12 family
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Gene
- Not found
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Sequence
- FLGELWNVWKSVF
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Sequence Length
- 13
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial
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Target Organism
-
- [Ref.27912176] Gram-negative bacteria: Escherichia coli (SBS 363)(MIC=12.5μmol/L);Pseudomonas aeruginosa (ATCC 27853)(MIC=3.12μmol/L);Escherichia coli (D31)(MIC=6.25μmol/L);Serratia marcescens (ATCC 4112)(MIC=3.12μmol/L);Enterobacter cloacae (beta-12)(MIC=50μmol/L);
- Gram-positive bacteria: Micrococcus luteus (A270)(MIC=3.12μmol/L);Staphylococcus aureus (ATCC 29213)(MIC=12.5μmol/L);Bacillus subtilis (ATCC 6633)(MIC=12.5μmol/L);Staphylococcus epidermidis (ATCC 12228)(MIC=6.25μmol/L);
- Fungi: Candida albicans MDM8(MIC=6.25μmol/L);Candida tropicalis (IOC 4560)(MIC=12.5μmol/L);
- Cryptococcus neoformans(MIC=6.25μmol/L)
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Hemolytic Activity
-
- [Ref.27912176] MHC=1.60μmol/L against human red blood cells
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Helical structures
-
Structure Description
- The peptide adopts Helical fraction 0.01 in water;0.11 in TFE/Water;0.24 in POPC;0.14 in POPC:POPG;0.23 in POPC:DOPE;
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20953.
Physicochemical Information
-
Formula
- C82H113N17O18
Absent Amino Acids
- ACDHIMPQRTY
Common Amino Acids
- FLVW
Mass
- 1624.9
PI
- 6
Basic Residues
- 1
Acidic Residues
- 1
Hydrophobic Residues
- 8
Net Charge
- 0
-
Boman Index
- 708
Hydrophobicity
- 0.592
Aliphatic Index
- 104.62
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 11000
Absorbance 280nm
- 916.67
Polar Residues
- 3
DRAMP20953
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria and fungi.It analog displayed a reduction in the antimicrobial activity, being the exceptions Escherichia coli SBS 363, Bacillus subtilis and Candida albicans.
Literature Information
- ·Literature 1
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Title
- Novel designed VmCT1 analogs with increased antimicrobial activity.
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Pubmed ID
- 27912176
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Reference
- Eur J Med Chem. 2017 Jan 27;126:456-463.
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Author
- Pedron CN, Torres MT, Lima JADS, Silva PI, Silva FD, Oliveira VX.