General Information

    • DRAMP ID

    • DRAMP20960

    • Peptide Name

    • Hp1404-T1d

    • Source

    • Synthetic construct

    • Family

    • Derived from Hp1404

    • Gene

    • Not found

    • Sequence

    • ILKKLLKKVKSI

    • Sequence Length

    • 12

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.29379033] Gram-negative bacteria: Pseudomonas aeruginosa ATCC 27853 (MIC=1.56 μM), Pseudomonas aeruginosa 138 (MIC=12.5 μM), Pseudomonas aeruginosa 431 (MIC=3.13 μM), Pseudomonas aeruginosa 434 (MIC=3.13 μM), Pseudomonas aeruginosa 557 (MIC=3.13 μM), Pseudomonas aeruginosa 559 (MIC=12.5 μM), Pseudomonas aeruginosa 778 (MIC=3.13 μM), Pseudomonas aeruginosa 1034 (MIC=3.13 μM), Pseudomonas aeruginosa 1162 (MIC=1.56 μM), Pseudomonas aeruginosa 3290 (MIC=6.25 μM), Pseudomonas aeruginosa 3399 (MIC=1.56 μM), Pseudomonas aeruginosa 3543 (MIC=25 μM), Pseudomonas aeruginosa 3592 (MIC=3.13 μM), Pseudomonas aeruginosa 3904 (MIC=6.25 μM), Pseudomonas aeruginosa 4007 (MIC=6.25 μM), Pseudomonas aeruginosa 4319 (MIC=3.13 μM), Pseudomonas aeruginosa 4891 (MIC=3.13 μM), Pseudomonas aeruginosa 5018 (MIC=1.56 μM), Pseudomonas aeruginosa 671973 (MIC=1.56 μM)

    • Hemolytic Activity

      • [Ref.29379033] Hp1404-T1d does not induce substantial hemolysis, even at the high tested concentration (200 μM) against mouse red blood cells

    • Cytotoxicity

      • [Ref.29379033] The cell survial against HaCaT cells is 58%, 48%, 52% and 34% at peptide concentrations of 25, 50, 100 and 200 μM

    • Binding Target

    • Lipopolysaccharide (LPS)-binding

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • The peptide adopts an alpha-helical conformation in the membrane-mimicking environments and adopts a random coil conformation in the presence of all liposome types .

    • Helical Wheel Diagram

    • DRAMP20960 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20960.

Physicochemical Information

    • Formula

    • C68H131N17O14

    • Absent Amino Acids

    • ACDEFGHMNPQRTWY

    • Common Amino Acids

    • K

    • Mass

    • 1410.89

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +5

    • Boman Index

    • -251

    • Hydrophobicity

    • 0.358

    • Aliphatic Index

    • 186.67

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP20960

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Mechanisms driving the antibacterial and antibiofilm properties of Hp1404 and its analogue peptides against multidrug-resistant Pseudomonas aeruginosa.

    • Reference

    • Sci Rep. 2018 Jan 29;8(1):1763.

    • Author

    • Kim MK, Kang HK, Ko SJ, Hong MJ, Bang JK, Seo CH, Park Y.