General Information

    • DRAMP ID

    • DRAMP20963

    • Peptide Name

    • Cp1 alpha s1-casein peptide derivative

    • Source

    • Synthetic construct

    • Family

    • Derived from bovine alpha S1-casein

    • Gene

    • Not found

    • Sequence

    • LRLKKYKVPQL

    • Sequence Length

    • 11

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, low hemolytic and toxic effects

    • Target Organism

      • [Ref.29783753] Gram-negative bacteria: Escherichia coli (ATCC 25922)(MIC=64μM);Escherichia coli (UB1005)(MIC=128μM);Salmonella pullorum (C7913)(MIC=256μM);Salmonella enterica subsp enterica (CMCC 50071)(MIC=256μM);
      • Gram-positive bacteria: Staphylococcus aureus (ATCC 29213)(MIC=640μM);L. monocytogenes (CMCC 54004)(MIC=64μM)

    • Hemolytic Activity

      • [Ref.2978375]Hemolysis 0% at 16μM;Hemolysis 23.54% at 512μM against human red blood cells

    • Cytotoxicity

      • [Ref.29783753] In 293 cells, The peptide had no cytotoxic activity at concentrations of 1-512 μM. The cell survival rates are 100%, 96%, 98%, 105%, 104%, 112%, 116%, 114% and 103% at concentrations of 1, 2, 4, 8, 16, 32, 64, 128, 256, 512 μM

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Unordered conformation

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20963 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20963.

Physicochemical Information

    • Formula

    • C66H116N18O14

    • Absent Amino Acids

    • ACDEFGHIMNSTW

    • Common Amino Acids

    • KL

    • Mass

    • 1385.76

    • PI

    • 10.46

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +4

    • Boman Index

    • -1845

    • Hydrophobicity

    • -0.636

    • Aliphatic Index

    • 132.73

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1490

    • Absorbance 280nm

    • 149

    • Polar Residues

    • 1

DRAMP20963

Comments Information

    • Function

    • Antibacterial activity against the Gram-positive and Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity against Human Cells of a Peptide Derived from Bovine

    • Reference

    • Molecules. 2018 May 19;23(5). pii: E1220.

    • Author

    • Hou J, Liu Z, Cao S, Wang H, Jiang C, Hussain MA, Pang S