General Information
-
DRAMP ID
- DRAMP20964
-
Peptide Name
- Synthesized Cecropin A (1–8)–LL37 (17–30) (C–L)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- KWKLFKKIFKRIVQRIKDFLRN
-
Sequence Length
- 22
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.29920061] Gram-negative bacteria: Escherichia coli (CVCC 245)(MIC=7.0 ± 0.21μg/mL);
- Gram-positive bacteria: Staphylococcus aureus (ATCC 25923)(MIC=2.0 ± 0.02μg/mL );L. mono. (CVCC 1599)(MIC=2.2 ± 0.05μg/mL)
-
Hemolytic Activity
-
- [Ref.29920061]HC50 =219 ± 2.98μg/mL against sheep erythrocyte cells
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20964.
Physicochemical Information
-
Formula
- C140H230N40O27
Absent Amino Acids
- ACEGHMPSTY
Common Amino Acids
- K
Mass
- 2905.62
PI
- 11.76
Basic Residues
- 9
Acidic Residues
- 1
Hydrophobic Residues
- 10
Net Charge
- +8
-
Boman Index
- -5905
Hydrophobicity
- -0.664
Aliphatic Index
- 101.82
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 261.9
Polar Residues
- 1
DRAMP20964
Comments Information
Function
- Antibacterial activity against the Gram-positive and Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Expression, Purification, and Characterization of a Novel Hybrid Peptide with Potent Antibacterial Activity.
-
Pubmed ID
- 29920061
-
Reference
- Molecules. 2018 Jun 20;23(6). pii: E1491.
-
Author
- Wei X, Wu R, Zhang L, Ahmad B, Si D, Zhang R.