General Information
-
DRAMP ID
- DRAMP20967
-
Peptide Name
- andricin 01 (Andrias davidianus, Amphibians, Animals)
-
Source
- Andrias davidianus
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- AIGHCLGATL
-
Sequence Length
- 10
-
UniProt Entry
- No entry found
-
Protein Existence
- Not found
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.28461043] Gram-positive bacteria : Bacillus subtilis CICC 10034 (MIC=32 μg/ml;MBC=32 μg/ml), Bacillus megaterium CICC 10448 (MIC=8 μg/ml;MBC=16 μg/ml), Staphylococcus aureus CICC 10306 (MIC=32 μg/ml;MBC=32 μg/ml), Staphylococcus aureus CICC 10384 (MIC=32 μg/ml;MBC=32 μg/ml), Listeria innocua CICC 10417 (MIC=32 μg/ml;MBC=32 μg/ml);
- Gram-negative bacteria : Escherichia coli CICC 10293 (MIC=8 μg/ml;MBC=16 μg/ml), Escherichia coli CGMCC 3373 (MIC=8 μg/ml;MBC=16 μg/ml), Escherichia coli CICC 10302 (MIC=8 μg/ml;MBC=16 μg/ml), Escherichia coli CICC 10300 (MIC=8 μg/ml;MBC=16 μg/ml), Pseudomonas aeruginosa CICC 21636 (MIC=4 μg/ml;MBC=8 μg/ml), Alcaligenes faecalis ATCC 8750 (MIC=8 μg/ml;MBC=16 μg/ml), Serratia marcescens ATCC 4112 (MIC=8 μg/ml;MBC=8 μg/ml), Enterobacter cloacae CICC 21539 (MIC=4 μg/ml;MBC=8 μg/ml), Salmonella Paratyphi CICC 10437 (MIC=8 μg/ml;MBC=16 μg/ml)
-
Hemolytic Activity
-
- [Ref.28461043] 0% hemolysis at 25 μg/ml , 0% hemolysis at 50 μg/ml , 4.3 ± 0.16% hemolysis at 100 μg/ml , 16.3 ± 2.16% hemolysis at 200 μg/ml against human hepatocyte cell line and human renal epithelial cells;0% hemolysis at 0–50 μg/ml against human red blood cells
-
Cytotoxicity
-
- [Ref.28461043] The cell viability of HL-7702 cells is 100.0±0.30%, 100.5±0.52%, 86.8±2.10% and 52.1±1.81% at peptide concentrations of 25, 50, 100 and 200 μg/ml.
- The cell viability of HREpiC cells is 100.1±0.20%, 103.1±1.20%, 82.3±1.84% and 50.3±2.05%
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Random coil
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP20967.
Physicochemical Information
-
Formula
- C41H70N12O12S
Absent Amino Acids
- DEFKMNPQRSVWY
Common Amino Acids
- AGL
Mass
- 955.14
PI
- 6.78
Basic Residues
- 1
Acidic Residues
- 0
Hydrophobic Residues
- 5
Net Charge
- +1
-
Boman Index
- 1431
Hydrophobicity
- 1.35
Aliphatic Index
- 137
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 4
DRAMP20967
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique
-
Pubmed ID
- 28461043
-
Reference
- Int J Antimicrob Agents.?2017 Jul;50(1):41-46. doi: 10.1016/j.ijantimicag.2017.02.013.
-
Author
- Jinjin Pei, Lei Jiang