• • DRAMP ID

  • DRAMP20968

• • Peptide Name

  • Catesbeianin-1 (Ranidae, Anura, Amphibia, Animals)

• • Source

  • Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • MMRVMRRKTKVIWEKKDFIGLYSID

• • Sequence Length

  • 25

• • UniProt Entry

  • C5IAX4

• • Protein Existence

  • Transcript level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.28258530] Gram-positive bacteria : Staphylococcus aureus ATCC 25923 (MIC=3.1 μg/ml;MBC=6.3 μg/ml), B. subtilis ATCC6633 (MIC=25 μg/ml;MBC=50 μg/ml), Streptococcus suis CVCC606 (MIC=3.1 μg/ml;MBC=12.5 μg/ml), Listeria monocytogenes ATCC19115 (MIC=12.5 μg/ml;MBC=25 μg/ml);
    • Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=3.1 μg/ml;MBC=12.5 μg/ml), Pseudomonas aeruginosa ATCC227853 (MIC=12.5 μg/ml;MBC=25 μg/ml), Salmonella spp. CVCC 3377 (MIC=6.3 μg/ml;MBC=25 μg/ml), Klebsiella pneumoniae H507 (MIC=25 μg/ml;MBC=100 μg/ml)

• • Hemolytic Activity

  • • [Ref.28258530] 0% hemolysis at 0 μg/ml , hemolysis 3% at 25 μg/ml , 7% hemolysis at 50 μg/ml , 8% hemolysis at 100 μg/ml , 35% hemolysis at 200 μg/ml , 50% hemolysis at 300 μg/ml , 85% hemolysis at 400 μg/ml , 95% hemolysis at 500 μg/ml against human red blood cells

• • Cytotoxicity

  • • [Ref.28258530] The cell viability of HEK293T cells is 102%, 103%, 103%, 100%, 98% and 85% at peptide concentrations of 0, 20, 40, 60, 80 and 100 μg/ml.
    • The cell viability of HL-7200 cells is 107%, 106%, 99%, 95% and 88% at peptide concentrations of 0,

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix

• • Structure Description

  • Catesbeianin-1 is an amphi pathic a-helix and that the helical wheel is divided into two parts, namely, a hydrophobic face and a hydro philic face.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20968.

DRAMP20968

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

• ·Literature 1

• • Title

  • Catesbeianin-1, a novel antimicrobial peptide isolatedfrom the skin of Lithobates catesbeianus (American bullfrog)

• • Pubmed ID

  • 28258530

• • Reference

  • Biotechnol Lett. 2017 Jun;39(6):897-903. doi: 10.1007/s10529-017-2312-7.

• • Author

  • Huihui Xu . Yang Zhang . Xin Feng . Kunyuan Tie . Yuan Cao . Wenyu Han