General Information

    • DRAMP ID

    • DRAMP20970

    • Peptide Name

    • P3 (bovine cathelicidin, cattle, ruminant, mammals, animals)

    • Source

    • Bos taurus (Bovine)

    • Family

    • Belongs to the globin family.

    • Gene

    • HBA

    • Sequence

    • VNFKLLSHSLLVTLASHL

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.25753638] Gram-positive bacteria : Staphylococcus aureus ATCC29213 (MIC=25 μg/ml), Clinical Staphylococcus aureus (MIC=12.5 μg/ml);
      • Gram-negative bacteria : Escherichia coli ATCC25922 (MIC=12.5 μg/ml), Clinical Escherichia coli (MIC=12.5 μg/ml), Pseudomonas aeruginosa clinical strain (MIC=25 μg/ml);
      • Fungi : Candida albicans Clinical strain (MIC=25 μg/ml), Candida albicans ATCC 90029 (MIC=50 μg/ml)

    • Hemolytic Activity

      • [Ref.25753638] 3% hemolysis at 50 μg/ml , 5% hemolysis at 100 μg/ml , 7% hemolysis at 200 μg/ml , 7.5% hemolysis at 300 μg/ml , 8% hemolysis at 400 μg/ml against human red blood cells

    • Cytotoxicity

      • [Ref.25753638] The cell viability of HaCaT cell lines is 93% and 94% at peptide concentrations of 200 and 400 μg/ml. In kumning mice, the LD50 of P3 is 160 mg/kg for i.p. injection, while the LD50 of PMB(served as a control) is 50 mg/kg. No mortality w

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20970 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20970.

Physicochemical Information

    • Formula

    • C93H154N24O24

    • Absent Amino Acids

    • CDEGIMPQRWY

    • Common Amino Acids

    • L

    • Mass

    • 1992.39

    • PI

    • 8.74

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • 811

    • Hydrophobicity

    • 1.05

    • Aliphatic Index

    • 167.78

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 5

DRAMP20970

DRAMP20970 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans

Literature Information

  • ·Literature 1

    • Title

    • Potential of Novel Antimicrobial Peptide P3 from Bovine Erythrocytes and Its Analogs To Disrupt Bacterial Membranes In Vitro and Display Activity against Drug-Resistant Bacteria in a Mouse Model

    • Reference

    • Antimicrob Agents Chemother. 2015 May;59(5):2835-41. doi: 10.1128/AAC.04932-14.

    • Author

    • Qinghua Zhang, Yanzhao Xu, Qing Wang, Bolin Hang, Yawei Sun, Xiaoxiao Wei, Jianhe Hu