• • DRAMP ID

  • DRAMP20973

• • Peptide Name

  • JH-2 (Derived from P3)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • VRFKLLSHSLLVTLASHL

• • Sequence Length

  • 18

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

• • Target Organism

  • • [Ref.25753638] Gram-positive bacteria : Staphylococcus aureus ATCC29213 (MIC=12.5 μg/ml), Clinical Staphylococcus aureus (MIC=6.25 μg/ml);
    • Gram-negative bacteria : Escherichia coli ATCC25922 (MIC=6.25 μg/ml), Clinical Escherichia coli (MIC=12.5 μg/ml), Pseudomonas aeruginosa clinical strain (MIC=6.25 μg/ml);
    • Fungi : Candida albicans ATCC 90029 (MIC=25 μg/ml), Candida albicans Clinical strain (MIC=25 μg/ml)

• • Hemolytic Activity

  • • [Ref.25753638] 2% hemolysis at 50 [Ref.25753638] 2% hemolysis at 50 μg/ml , 2.5% hemolysis at 100 μg/ml , 3.5% hemolysis at 200 μg/ml , 4% hemolysis at 300 μg/ml , 4% hemolysis at 400 μg/ml against human red blood cells

• • Cytotoxicity

  • • [Ref.25753638] The cell viability of HaCaT cell lines is 88% and 84% at peptide concentrations of 200 and 400 μg/ml. In Kunming mice, the LD50 of JH-2 is 133 mg/kg for i.p. injection, while the LD50 of PMB(served as a control) is 50 mg/kg. no mortality

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP20973.

DRAMP20973

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans

• ·Literature 1

• • Title

  • Potential of Novel Antimicrobial Peptide P3 from Bovine Erythrocytes and Its Analogs To Disrupt Bacterial Membranes In Vitro and Display Activity against Drug-Resistant Bacteria in a Mouse Model

• • Pubmed ID

  • 25753638

• • Reference

  • Antimicrob Agents Chemother. 2015 May;59(5):2835-41. doi: 10.1128/AAC.04932-14.

• • Author

  • Qinghua Zhang, Yanzhao Xu, Qing Wang, Bolin Hang, Yawei Sun, Xiaoxiao Wei, Jianhe Hu