General Information
-
DRAMP ID
- DRAMP20974
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Peptide Name
- JH-3 (Derived from P3)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- RRFKLLSHSLLVTLASHL
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Sequence Length
- 18
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal
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Target Organism
-
- [Ref.25753638]Gram-positive bacteria : Staphylococcus aureus ATCC29213 (MIC=6.25 μg/ml), Clinical Staphylococcus aureus (MIC=3.125 μg/ml);
- Gram-negative bacteria : Escherichia coli ATCC25922 (MIC=3.125 μg/ml), Clinical Escherichia coli (MIC=3.125 μg/ml), Pseudomonas aeruginosa clinical strain (MIC=6.25 μg/ml);
- Fungi : Candida albicans ATCC 90029 (MIC=12.5 μg/ml), Candida albicans Clinical strain (MIC=6.25 μg/ml)
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Hemolytic Activity
-
- [Ref.25753638] 8% hemolysis at 50 μg/ml , 13% hemolysis at 100 μg/ml , 17% hemolysis at 200 μg/ml , 18% hemolysis at 300 μg/ml , 18.5% hemolysis at 400 μg/ml against human red blood cells
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Cytotoxicity
-
- [Ref.25753638] The cell viability of HaCaT cell lines is 86% and 86% at peptide concentrations of 200 and 400 μg/ml. In Kunming mice, the LD50 of JH-3 is 180 mg/kg for i.p. injection, , while the LD50 of PMB(served as a control) is 50 mg/kg. No mortali
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Binding Target
- Not found
Structure Information
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Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Free
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP20974.
Physicochemical Information
-
Formula
- C96H163N29O23
Absent Amino Acids
- CDEGIMNPQWY
Common Amino Acids
- L
Mass
- 2091.53
PI
- 12.01
Basic Residues
- 5
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +5
-
Boman Index
- -1913
Hydrophobicity
- 0.511
Aliphatic Index
- 151.67
Half Life
-
- Mammalian:1 hour
- Yeast:2 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 4
DRAMP20974
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans
Literature Information
- ·Literature 1
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Title
- Potential of Novel Antimicrobial Peptide P3 from Bovine Erythrocytes and Its Analogs To Disrupt Bacterial Membranes In Vitro and Display Activity against Drug-Resistant Bacteria in a Mouse Model
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Pubmed ID
- 25753638
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Reference
- Antimicrob Agents Chemother. 2015 May;59(5):2835-41. doi: 10.1128/AAC.04932-14.
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Author
- Qinghua Zhang, Yanzhao Xu, Qing Wang, Bolin Hang, Yawei Sun, Xiaoxiao Wei, Jianhe Hu