General Information

    • DRAMP ID

    • DRAMP20976

    • Peptide Name

    • adevonin (Derived from Adenanthera pavonina trypsin inhibitor (ApTI)) inhibitor (ApTI))

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RKYVRFLHRWVKYFRAYL

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.30570384]Gram-positive bacteria : Staphylococcus aureus ATCC 80958 (MIC=7.35 μM);
      • Gram-negative bacteria : Escherichia coli ATCC 35218 (MIC=7.35 μM), Klebsiella oxytoca ATCC 13182 (MIC=1.84 μM), Klebsiella pneumoniae ATCC 70603 (MIC=3.67 μM), Serratia marcesens ATCC 13880 (MIC=1.84 μM), Klebsiella pneumoniae KpC+ 001825971 (MIC=25.6 μM)

    • Hemolytic Activity

      • [Ref.30570384] 0% hemolysis at 43.75 μM , 72.5% hemolysis at 175 μM , 77% hemolysis at 350 μg/ml against human type O erythrocytes

    • Cytotoxicity

      • [Ref.30570384] In fibroblasts, a significant reduction of cell viability, of 25% on average, is observed in each time interval(24,48 and 72h) in the highest peptide concentration(10 μM)

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Adevonin showed that its secondary structure varies according to the environment, acquiring

    • Helical Wheel Diagram

    • DRAMP20976 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20976.

Physicochemical Information

    • Formula

    • C123H181N35O22

    • Absent Amino Acids

    • CDEGIMNPQST

    • Common Amino Acids

    • R

    • Mass

    • 2502.01

    • PI

    • 11.05

    • Basic Residues

    • 7

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +7

    • Boman Index

    • -4784

    • Hydrophobicity

    • -0.578

    • Aliphatic Index

    • 81.11

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 9970

    • Absorbance 280nm

    • 586.47

    • Polar Residues

    • 3

DRAMP20976

DRAMP20976 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Adevonin, a novel synthetic antimicrobial peptide designed from the Adenanthera pavonina trypsin inhibitor (ApTI) sequence

    • Reference

    • Pathog Glob Health.?2018 Dec;112(8):438-447. doi: 10.1080/20477724.2018.1559489.

    • Author

    • Mayara S. Rodrigues, Caio F. R. de Oliveira, Luís H. O. Almeida, Simone M.Neto, Ana Paula A. Boleti, Edson L. dos Santos, Marlon H. Cardoso, Suzana,M. Ribeiro, Octávio L. Franco, Fernando S. Rodrigues, Alexandre J. Macedo,Flávia R. Brust & Maria Lígia R. Macedo