General Information

    • DRAMP ID

    • DRAMP20985

    • Peptide Name

    • CPF-4 (Derived from CPF-C1)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • kFWKlLKkAlrLWAkVL

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.28371431]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=16 μg/ml), Staphylococcuss aureus 725 (MIC=16 μg/ml), Staphylococcuss aureus 794 (MIC=8 μg/ml), Staphylococcuss aureus 936 (MIC=8 μg/ml);
      • Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=16 μg/ml), Escherichia coli 780 (MIC=16 μg/ml), Escherichia coli 804 (MIC=16 μg/ml), Escherichia coli 850 (MIC=8 μg/ml), Pseudomonas aeruginosa ATCC 27853 (MIC=16 μg/ml), Pseudomonas aeruginosa 2760 (MIC=8 μg/ml), Pseudomonas aeruginosa 9132 (MIC=32 μg/ml), Acinetobacter baumannii ATCC 19606 (MIC=8 μg/ml), Acinetobacter baumannii 1980 (MIC=16 μg/ml), Acinetobacter baumannii 2982 (MIC=16 μg/ml), Acinetobacter baumannii 4956 (MIC=16 μg/ml)

    • Hemolytic Activity

      • [Ref.28371431] 0% hemolysis at 32 μg/ml , 5% hemolysis at 64 μg/ml , 8% hemolysis at 128 μg/ml , 10% hemolysis at 256 μg/ml , 25% hemolysis at 512 μg/ml against human red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • Mixed(D-Lys1, D-Leu5, D-Lys8, D-Leu10, D-Arg11, D-Lys15)

    • Structure

    • Random coil

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20985 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20985.

Physicochemical Information

    • Formula

    • C108H175N27O17

    • Absent Amino Acids

    • CDEGHIMNPQSTY

    • Common Amino Acids

    • KL

    • Mass

    • 2141.76

    • PI

    • 11.39

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +6

    • Boman Index

    • -277

    • Hydrophobicity

    • 0.211

    • Aliphatic Index

    • 135.56

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 647.06

    • Polar Residues

    • 0

DRAMP20985

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Novel Antimicrobial Peptide CPF-C1 Analogs with Superior Stabilities and Activities against Multidrug-Resistant Bacteria

    • Reference

    • Chem Biol Drug Des. 2017 Nov;90(5):690-702. doi: 10.1111/cbdd.12988.

    • Author

    • Junqiu Xie,Qian Zhao,Sisi Li,Zhibin Yan,Jing Li,Yao Li,Lingyun Mou,Bangzhi Zhang,Wenle Yang,Xiaokang Miao,Xianxing Jiang,and Rui Wang