General Information
-
DRAMP ID
- DRAMP21003
-
Peptide Name
- [K8R]cGm (Derived from Gm)
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GCRRLCYRQRCVTYCRGR
-
Sequence Length
- 18
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antitumor
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Target Organism
-
- [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=16 μM);
- Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=1-2 μM)
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Hemolytic Activity
-
- [Ref.28741926] 34.8-54.8% hemolysis at 64 μM against human red blood cells
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Cytotoxicity
-
- [Ref.28741926] CC50 = 29.4 ± 1.1 μM against CRL-1739, CC50 = 5.0 ± 0.3 μM against MM96L, CC50 = 34.5 ± 3.6 μM against HFF-1, CC50 = 39.4 ± 2.6 μM against HeLa, CC50 = 3.1 ± 0.1 μM against K-562.
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- No specific N-terminal
-
C-terminal Modification
- No specific C-terminal
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys2 and Cys15, Cys6 and Cys11.
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21003.
Physicochemical Information
-
Formula
- C90H153N37O23S4
Absent Amino Acids
- ADEFHIKMNPSW
Common Amino Acids
- R
Mass
- 2249.68
PI
- 10.16
Basic Residues
- 6
Acidic Residues
- 0
Hydrophobic Residues
- 2
Net Charge
- +6
-
Boman Index
- -8195
Hydrophobicity
- -0.922
Aliphatic Index
- 37.78
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 3230
Absorbance 280nm
- 190
Polar Residues
- 9
DRAMP21003
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria
Literature Information
- ·Literature 1
-
Title
- Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties
-
Pubmed ID
- 28741926
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Reference
- ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459.?
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Author
- Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik