General Information

    • DRAMP ID

    • DRAMP21005

    • Peptide Name

    • [R4A, R18A]cGm (Derived from Gm)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GCRALCYKQRCVTYCRGA

    • Sequence Length

    • 18

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antitumor

    • Target Organism

      • [Ref.28741926]Gram-positive bacteria : Staphylococcuss aureus ATCC 25923 (MIC=32 μM);
      • Gram-negative bacteria : Escherichia coli ATCC 25922 (MIC=8 μM), Escherichia coli CGSC 5167 (MIC=0.5-1 μM), Klebsiella pneumoniae ATCC 700603 (MIC=32 μM), Acinetobacter baumannii ATCC 19606 (MIC=8-16 μM), Pseudomonas aeruginosa ATCC 27853 (MIC=8 μM), Helicobacter pylori ATCC 43504 (MIC>80 μM);
      • Fungi : Candida albicans ATCC 90028 (MIC=32 μM), Cryptococcus neoformans ATCC 208821 (MIC=8-16 μM)

    • Hemolytic Activity

      • [Ref.28741926] 0% hemolysis at 17 μM , 3% hemolysis at 35 μM , 30-48.6% hemolysis at 64 μM , 40% hemolysis at 100 μM against human red blood cells

    • Cytotoxicity

      • [Ref.28741926] CC50 > 64 μM against CRL-1739, CC50 = 10.3 ± 1.1 μM against MM96L, CC50 = 51.2 ± 4.0 μM against HFF-1, CC50 > 64 μM against HeLa, CC50 = 48.4 ± 0.7 μM against MCF-7, CC50 = 11.5 ± 0.6 μM against K-562, CC50 = 34.1 ± 3.9 μM against HL-60;CC50=30.8 ± 2.5μM against PBMCs.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys2 and Cys15, Cys6 and Cys11.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21005 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21005.

Physicochemical Information

    • Formula

    • C84H139N29O23S4

    • Absent Amino Acids

    • DEFHIMNPSW

    • Common Amino Acids

    • C

    • Mass

    • 2051.45

    • PI

    • 9.25

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +4

    • Boman Index

    • -3912

    • Hydrophobicity

    • -0.189

    • Aliphatic Index

    • 48.89

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 3230

    • Absorbance 280nm

    • 190

    • Polar Residues

    • 9

DRAMP21005

DRAMP21005 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria, Antifungal activity agaist Candida albicans, Cryptococcus neoformans

Literature Information

  • ·Literature 1

    • Title

    • Redesigned Spider Peptide with Improved Antimicrobial and Anticancer Properties

    • Reference

    • ACS Chem Biol. 2017 Sep 15;12(9):2324-2334. doi: 10.1021/acschembio.7b00459.?

    • Author

    • Sonia Troeira Henriques, Nicole Lawrence, Stephanie Chaousis, Anjaneya S. Ravipati, Olivier Cheneval,Aurelie H. Benfield, Alysha G. Elliott, Angela Maria Kavanagh, Matthew A. Cooper, Lai Yue Chan,Yen-Hua Huang, and David J. Craik